Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 20, Problem 4A

The following data were obtained from a disk-diffusion test.

Antibiotic Zone of Inhibition
A 15 mm
B 0 mm
c 7 mm
D 15 mm
  1. a. Which antibiotic was most effective against the bacteria being tested?
  2. b. Which antibiotic would you recommend for treating a disease caused by this bacterium?
  3. c. Was antibiotic A bactericidal or bacteriostatic? How can you tell?
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What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating")  bacteria, and what can we use them for? Check all that apply   A. To observe colony morphology/color   B. To isolate viral plaques   C. testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,)   D. To isolate a clonal population of bacteria from the rest of the culture   E. For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly)   F. Accurate quantification (to calculate CFU/mL)   G. To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate.   H. To lower risk of contamination/identify contamination
Based on the following image, which concentration of antibiotic would be considered the minimum bactericidal concentration (MBC)? a. 8 ug/mlb. 16 ug/mlc. 25 ug/ml
After an antimicrobial drugs test, the following results were obtained from a disk-diffusion test against a bacterium.   Antiboitic A= 6mm Antibiotic B= 18mm Antibiotic C= 11mm Antibiotic D= 19mm which drug should be used to treat an infect caused by this bacterium (assuming everything else is equal about these antibiotics)?
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