Biological Science (6th Edition)
Biological Science (6th Edition)
6th Edition
ISBN: 9780321976499
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Emily Taylor, Greg Podgorski, Jeff Carmichael
Publisher: PEARSON
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Chapter 20, Problem 2TYK

What is a plasmid?

a. an organelle found in many bacteria and certain eukaryotes

b. a circular DNA molecule that replicates independently of the main chromosome(s)

c. a type of virus that has a DNA genome and infects certain types of human cells, including lung and respiratory tract tissue

d. a type of virus that has an RNA genome, codes for reverse transcriptase, and inserts a cDNA copy of its genome into cells

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What is a proteome? a. The collection of all genes encoding proteins b. The collection of all proteins encoded by the genome c. The collection of all proteins present in a cell d. The amino acid sequence of a protein
How does a retrovirus prepare its genetic material for incorporation into the host cell genome?   a. Host enzymes incorporate viral RNA directly into the genome.   b. Host enzymes covert viral RNA to double-stranded viral DNA.   c. Viral reverse transcriptase uses viral RNA to make double-stranded viral RNA.   d. Viral reverse transcriptase uses viral RNA to make double-stranded viral DNA.   e. Viral reverse transcriptase uses viral single-stranded DNA to make double-stranded viral DNA.
Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.
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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license