The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

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Answer 1

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Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

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In a typical PCR reaction what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.

The final amount of each primer required in a PCR reaction is 25 picomol. If the total volume of the PCR reaction is equal to 100 µl and the stock concentration of each primer is equal to 0.0025 mM. Calculate the volume of stock primer that needs to be added in order to ensure a primer amount of 25 picomol.

Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples didn’t already contain dye and you wanted to load your PCR sample onto an agarose gel, you’d need to add loading dye to the proper concentration. There is a 6X loading dye available for use; how many µl of this loading dye will you add to 10 µl of your sample so that it is at a 1X working concentration? Show your work.

Answer 2

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

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Answer 3

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

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Answer 4

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Expert Solution & Answer

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Answer 5

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 6

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Answer 7

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Answer 8

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

Answer 9

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

Answer 10

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 11

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 12

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Answer 13

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Answer 14

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Answer 15

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The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Solution Summary: The author explains the polymerase chain reaction, which produces multiple copies of a specific DNA segment.

Videos

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Want to see the full answer?

Chapter 20 Solutions

The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Solution Summary: The author explains the polymerase chain reaction, which produces multiple copies of a specific DNA segment.

Videos

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Want to see the full answer?

Chapter 20 Solutions

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.