Concept explainers
Genetic Analysis
Chi
Want to see the full answer?
Check out a sample textbook solutionChapter 20 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
- Info:(For Wildtype) Extinction Coefficient BGG: 0.0007 (y = 0.0007x Beer's Law Graph) Extinction Coefficient BSA: 0.0011 (y = 0.0011x Beer's Law Graph) Cell Lysate: ABS: 0.2, BSA Protein: 285.7mM, BGG Protein: 181.8mM Cell Lysate 1 in 5 dilution: ABsorbtion, 0.03, BSA Protein: 214.3mM, BGG Protein: 136.4mM Elution: Abs .03, Protein BSA: 42.9mM, Protein BGG: 27.3mM Elution DIlution 1/5: ABS: 0.005, Protein BSA: 35.7mM, Protein BGG: 22.7mM Problem: 5. Looking at the sequence of the BGG and BSA standards compared to the sequence of MBP-BAP, which ofthe two standards is the better choice for determining the concentration of MBP-BAP. A. What amino acids react with the Bradford reagent? B. Find the % Composition of amino acids that react with Bradford C. Which standard is best for determining the concentration of MBP-BAP? Briefly explain. D. Explain briefly why choosing a specific standard is less important for determining the concentration of proteinin the cell lysate compared to the…arrow_forward3.17 The accompanying pedigree and gel diagram phenotypes of the progeny, and in what propor- show the phenotypes of the parents for an SSRP that has multiple alleles. What are the possible thdt that has multiple alleles. What are the possible phenotypes of the progeny, and in what propor. tions are they expected? bobiah of 152 148 144 140 Be %3D eekinarrow_forwardCodominance ABO blood typing is an example of codominance, where A and B are both dominant to O. A woman who is AB type has a child with a man who is O type. Use I allele formatting to designate each genotypes: IA IA, IA i, IB IB, IB i, IA IB, ii A) List the genotype of each parent. B) Complete a Punnett square. List all the genotypes for the predicted offspring of this mating (using the format of genotypes listed above) C) What is the probability that any of their offspring will be O type? D) What is the probability that any of their offspring will be AB type?arrow_forward
- Give nine examples of lethal alleles in humans, other animals, and plants and a brief molecular explanation (i.e., why are they lethal).arrow_forward3 (a). The S-s antigen system in human is controlled by two codominant alleles, S and s. In a group of 3146 individuals, the following genotypic frequencies were found: 188 SS, 717 Ss, and 2241 ss. Calculate the frequency of S. O p = 0.3474 O p = 0.2877 O p = 0.1737 O p = 0.1438arrow_forwardstion 6 of 18 Suppose that a geneticist discovers a new mutation in Drosophila melanogaster that causes the flies to shake and quiver. She calls this mutation quiver, qu, and determines that it is due to an autosomal recessive gene. She wants to determine whether the gene encoding quiver is linked to the recessive gene for vestigial wings, vg. She crosses a fly homozygous for quiver and vestigial traits with a fly homozygous for the wild-type traits, and then uses the resulting F, females in a testcross. She obtains the flies from this testcross. Phenotype Number of flies vg* qu+ 230 vg qu 224 vg qut vg* qu 97 99 Test the hypothesis that the genes quiver and vestigial assort independently by calculating the chi-squared, X², for this hypothesis. Provide the X2 to one decimal place. X2 = Does the X value support the hypothesis that the quiver and vestigial genes assort independently? Why or why not? the partial table of critical values for X2 calculations to test this hypothesis.arrow_forward
- Explain this statement "Major considerations in dosage form design with example" ? Please briefly explain at your own easy words.arrow_forwardSearch the menus (Alt+/) 75% Normal text Calibri в IUA 11 + ... 1.| 2 | 3 | • 4.| 5 6 8 12. Although Laurlanthalasa, Princess of the Qualinesti elves, is known for riding into battle on a silver dragon, this is not her main form of transportation. She generally travels by way of griffon. Although the allele for a "crown" of feathers around the head is dominant, it is rarely found in the wild. This may be due to the fact that it makes the griffons stand out, thus making them less effective hunters and more visible to their natural enemies, evil chromatic dragons. This scarcity also makes them prized by elven royalty. Lauralanthalasa was given a breeding pair of bald griffons by an admirer who claims that they are both hybrids for the crowned gene. Create a Punnett Square to determine if Lauralanthalasa will have any chance using these two griffins to produce crowned offspring. Alleles (letters) and Phenotypes All Genotype and Phenotype Parent Punnett Square Possibilities Genotypes…arrow_forwardThe following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular weight. a. If the tracking dye had moved a distance of 90.0 mm, calculate linear regression parameters such as linearity coefficient (r), the slope (m), and the y-intercept (b). Protein MW (Da Distance moved (mm) Transferrin 78 000 6.0 Bovine serum albumin 66 000 12.5 Ovalbumin 45 000 32.0 54.0 Trypsinogen Myoglobin Lysozyme Cytochrome c 24 000 17 800 69.0 14 300 79.0 12 400 86.5 Proliferating cell nuclear antigen (PCNA) acts as a homotrimer of three identical subunits and helps increase the processivity of leading strand synthesis during DNA replication. It has a molecular weight of 36.0 kDa. Under the reducing conditions of the sample buffer, the disulfide bridges linking the subunits are reduced and thus the monomer chains are seen on the gel. b. Calculate the Rf of the single band of PCNA when subjected to SDS-PAGE. c. Predict the distance (in mm)…arrow_forward
- 1. DO NOT ROUND OFF YOUR ANSWERS PREMATURELY. Use the unrounded and complete values when computing for the genetic gain; 2. Upon encoding, all final answers should be rounded-off to TWO decimal places. Examples: 3.5798 = 3.58, 0.0079 = 0.01, 0.00000008 = 0.00; 2. Report your answers as NUMERIC VALUES only. Please exclude the units; 3. Write ONLY one answer for each question. Avoid using "or", "/", "&" and "and". Provide only what is asked to you; 4. AVOID using unnecessary PUNCTUATIONS (. , ? 1; ; etc.), SYMBOLS ( []{ } etc.) and SPACES (before or after your answer). Please follow these instructions carefully. Prediction of Genetic Gain. If the milk yield of a herd of cow is 6,479 kg while those kept for breeding is 6,890 kg, and the h2 estimate of milk yield is 0.85. And the generation interval of dairy cattle is 4 years. What will be the expected genetic gain in the milk yield (kg/year) of the next generation?arrow_forward32 What is the probability that the offspring of a homozygous dominant individual and a homozygous recessive individual will exhibit the dominant phenotype? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 0.25 0.66 0.5 1.0arrow_forward1 (b). Nowadays, newborn babies are required to be tested for phynylketonuria (PKU), an autosomal recessive genetic disorder. If untreated, individual homozygous for PKU suffer mental retardation. In a recent year, 4 PKU babies were detected out of 126,o00 tested. Assuming Hardy-Weinberg conditions, What is the percentage of heterozygous carriers? (Give your answer in whole number, no decimal places, and include the % symbol without a space).arrow_forward