Prescott's Microbiology
11th Edition
ISBN: 9781260409062
Author: WILLEY, Joanne
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Question
Chapter 2, Problem 2AL
Summary Introduction
A photograph or a digital image that is taken with the help of a microscope is known as a micrograph or photomicrograph. A magnified image of the specimen is obtained from the micrograph. Micrography can be defined as the art or practice of using a light microscope to make photographs. It provides wide-spread details of the microstructure of the specimens.
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What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)
List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step.
1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage.
a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage.
4. Use the Low Power Objective.
a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
Chapter 2 Solutions
Prescott's Microbiology
Ch. 2.1 - Prob. 1MICh. 2.1 - Prob. 1CCCh. 2.1 - Prob. 2CCCh. 2.1 - Prob. 3CCCh. 2.2 - Prob. 1MICh. 2.2 - Prob. 2MICh. 2.2 - Prob. 3MICh. 2.2 - Prob. 1CCCh. 2.2 - Prob. 2CCCh. 2.2 - Prob. 3CC
Ch. 2.2 - Prob. 4CCCh. 2.2 - Prob. 5CCCh. 2.2 - Prob. 6CCCh. 2.3 - Prob. 1MICh. 2.3 - Prob. 1CCCh. 2.3 - Retrieve, Infer, Apply 2. Why would basic dyes be...Ch. 2.3 - Prob. 3CCCh. 2.3 - Prob. 4CCCh. 2.4 - Prob. 1MICh. 2.4 - Prob. 1CCCh. 2.4 - Prob. 2CCCh. 2.4 - Prob. 3CCCh. 2.4 - Prob. 4CCCh. 2.5 - Prob. 1MICh. 2.5 - Prob. 1CCCh. 2.5 - Prob. 2CCCh. 2 - Prob. 1RCCh. 2 - Prob. 2RCCh. 2 - Prob. 3RCCh. 2 - Prob. 1ALCh. 2 - Prob. 2ALCh. 2 - Prob. 4AL
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- The diffraction limit is an unbeatable barrier limiting the effective resolution of light microscopy due to the inherent wavelength of light.True or Falsearrow_forwardPlease provide the quality assurance procedure that ensures reliable results in CLINICAL MICROSCOPY? Please answer in detail; thank youarrow_forwardWhat factors must be addressed to properly acquire quantitative fluorescence microscopy images? What information about the imaging system should be recorded? What controls are needed and what standards are available?arrow_forward
- PROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep things easy. 2. Stick a long piece of tape over the two pencils and to the table on either side of the pencils to hold the tape tightly between the two pencils like a bridge. 3. Don’t touch the sticky side of the tape or you will ruin the microscope. Drop a small drop of water onto the top of the tape using the pipette or medicine dropper. 4. Make 3-4 lines of tape and add a different-sized drop to each one. This will help determine what size of water droplet produces the biggest magnification. 5. Prepare a rectangular shape of plastic cover. Put a small slice of onion. Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size of the onion on different droplets. Write your observations on the table below. No. of drops Observationarrow_forwardComplete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)arrow_forwardFill in the data table belowarrow_forward
- The following questions talk about increasing magnification in microscopes. Please answer questions 1 and 2 After a specimen is in focus, where in the field of view should position the specimen before increasing the magnification (be precise)? (QUESTION 2 HAS TWO PARTS TO IT): To increase the magnification, which objective should you click into position next? After refocusing the specimen with the new objective, if you want to increase the magnification even more, which objective should you now click into position?arrow_forwardCan you help me to fill in the function of each, pleasearrow_forwardWhat is the proper method of cleaning the microscope? Select an answer. a. Change the magnification to the lowest power, clean the objective lens and ocular lens with lens paper b. Change the magnification to the highest power, clean the objective lens and ocular lens with lens paper c. Change the magnification to the lowest power, clean the objective lens and ocular lens with a paper towelarrow_forward
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