Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 19.7, Problem 1CR
Why might a microarray be superior to using high-throughput sequencing to identify a rare population member in a complex microbial community? What are the advantages and limitations of FISH and PhyloChips for analysis of microbial communities?
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Chapter 19 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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- Enumerate 15 genus/species of bacteria that thrives on a rare microbial biosphere and provide the following details for each genus/species (prepare in table form): a. General characteristics b. Metabolic diversity (e.g. chemoorganotrophs, photoautotrophs, etc.) c. Most suitable methodological assessment (e.g. OTUs, ASVs, Metagenome Sequencing, etc.)arrow_forwardwhat was the driving force for the development of microscale testingmethods for ecotoxicology.arrow_forwardThe techniques for identifying unknown bacteria can be summarized in three key steps, explain in your own words how to: Staining the unknown for initial characterization by microscopy. Using a dichotomous key strategy to systematically rule out other organisms. Testing the organism for key biochemical traits.arrow_forward
- How could FISH (fluorescence in situ hybridization) be used to determine the relative proportions of archaea and bacteria in a population?arrow_forwardBriefly describe an irreversible and a reversible hypotheses to explain the loss of morphogenic potential for in vitro cultures.arrow_forwardDescribe in full the technique of density gradient centrifugation?arrow_forward
- Microbiomes can be characterized using a number of molecular biology approaches. Which approach would provide a better functional description of a community: metagenomic or metatranscriptomic sequencing? Briefly explain your reasoning.arrow_forwardHow has single-cell genomics contributed to newunderstanding of factors controlling the diversity ofProchlorococcus in the ocean?arrow_forwardWhy are molecular techniques important in studying microbial ecology?arrow_forward
- Why are live cell cultures better than preserved samples for microbial taxonomy?arrow_forwardRecall that while FOVS will change with changing objectives, the size of the organism itself does not change. Consider the next example. Example #1 Polyp Objective: 40x Example #2 Polyp blObjective: 10x Example #3 Polyp Objective: 4x Field of view: 500 µm Field of view: 2000 µm Field of view: 5000 µm Estimated # of Estimated # of Estimated # of organisms that fit organisms that fit organisms that fit across the FOV: 1 Approximate size of organism: 500μm/ 1 = 500 μm across the FOV: 4 across the FOV: 10 Approximate size of organism: 2000µm/ 4 = 500 µm Approximate size of organism: 5000μm/ 10-500 μm %3D The three examples above are of the same jellyfish polyp, but at varying objectives. What is the size of the polyp at the 40x objective? 10x? 4x? Which objective visualizes the polyp best? Which objective would be best used to estimate organism size with?arrow_forwardwhat are some of the strengths and weaknesses to using a Culture dependent enumeration approach from a microbial perspectivearrow_forward
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