Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 18.11, Problem 1MQ
- How can stable isotope probing reveal the identity of an organism that carries out a particular process?
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Determine the methods that could be used on a sample (obtained from the ocean of Jupiter's moon) to differentiate between a novel cellular life form and contamination of an organism from the Earth based on DNA, RNA, and protein.
Define ecotype. Do you think it is necessary to obtain microbes in pure culture before assigning different ecotypes? Explain.
What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for the enrichment of only a certain group or groups of organisms?
Chapter 18 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 18.1 - Describe the enrichment strategy behind...Ch. 18.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 18.1 - What is enrichment bias? How does dilution reduce...Ch. 18.2 - How does the agar dilution method differ from...Ch. 18.2 - How might you isolate a morphologically unique...Ch. 18.2 - What is meant by high-throughput in culturing...Ch. 18.3 - How does viability staining differ from stains...Ch. 18.3 - Prob. 2MQCh. 18.3 - Why is it incorrect to say that the GFP is a...Ch. 18.4 - What structure in the cell is the target for...
Ch. 18.4 - FISH and CARD-FISH can be used to reveal different...Ch. 18.5 - What could you conclude from PCR/DGGE analysis of...Ch. 18.5 - What surprising finding has come out of many...Ch. 18.6 - MINIQUIZ
• What is a phylochip and what can it...Ch. 18.6 - What are the advantages and disadvantages of...Ch. 18.6 - Prob. 3MQCh. 18.7 - Prob. 1MQCh. 18.7 - How do environmental genomic approaches differ...Ch. 18.7 - Prob. 3MQCh. 18.8 - Prob. 1MQCh. 18.8 - If a large pulse of organic matter entered the...Ch. 18.9 - Prob. 1MQCh. 18.9 - What is the simplest explanation for why lunar...Ch. 18.9 - What is the expected isotopic composition of...Ch. 18.10 - How could NanoSIMS be used to identify a...Ch. 18.10 - Prob. 2MQCh. 18.10 - How does MAR-FISH link microbial diversity and...Ch. 18.11 - How can stable isotope probing reveal the identity...Ch. 18.11 - What key method is required to do genomics on a...Ch. 18.11 - Prob. 3MQCh. 18 - Prob. 1RQCh. 18 - Prob. 2RQCh. 18 - Prob. 3RQCh. 18 - Prob. 4RQCh. 18 - Prob. 5RQCh. 18 - Prob. 6RQCh. 18 - Prob. 7RQCh. 18 - Prob. 8RQCh. 18 - Prob. 9RQCh. 18 - REVIEW QUESTIONS
10. Why is a microarray not...Ch. 18 - Prob. 11RQCh. 18 - Prob. 12RQCh. 18 - Q What are the major advantages of radioisotopic...Ch. 18 - Prob. 14RQCh. 18 - Prob. 15RQCh. 18 - REVIEW QUESTIONS
16. What is the advantage of...Ch. 18 - Prob. 17RQCh. 18 - Design an experiment for measuring the activity of...Ch. 18 - You wish to know whether Archaea exist in a lake...Ch. 18 - Design an experiment to solve the following...Ch. 18 - Design a SIP experiment that would allow you to...
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- What is meant by the term “enrichment culture”?arrow_forwardExplain, in your own words, why it is important to perform serial dilution when trying to quantify bacterial organisms.arrow_forwardDetermine the approaches that might be tried on a sample (obtained from ocean of Jupiter's moon) to distinguish between contamination of an organism from the Earth, and a novel cellular life-form on the basis of DNA, RNA, and protein. Don't reroute.arrow_forward
- What are advantages and disadvantages of solid phase bioremediation technique?arrow_forwardDescribe how would you determine the following using a real-time PCR instrument. A) The quantity and quality of a DNA sample. B) Whether an individual had cystic fibrosis from a genomic DNA sample. C) If several bacterial pathogens were present in DNA extracted from a food sample.arrow_forwardBriefly describe an irreversible and a reversible hypotheses to explain the loss of morphogenic potential for in vitro cultures.arrow_forward
- How reliable is PCR to detect microorganisms in water samples?arrow_forwardUsing the clinical question --"Does antibacterial foam decrease bacteria count on hands as much as hand washing with soap and water.” Question 1) Describe your study design and sample. 2) Will your findings be generalizable? Why or why not? 3) How does this exercise inform your interpretation of published research?.arrow_forwardCan gel electrophoresis be a quantitative technique? How so?arrow_forward
- Which of the following types of data produce curves similar in shape to the graph? Select all that apply 1.Bacterial growth in a culture 2.A normal distribution of height in a population of students 3.Exponential population growth 4.Haemoglobin binding of oxygen 5.Log transformations of drug dose curves 6.Osmotic fragility of red blood cells 7.A standard curve for an enyme assayarrow_forwardDefine the following termonologies in brief 1.Cellular Permeability 2.Fluorescence in situ Hybridization(FISH) 3. Genotoxicity 4.Medical Device 5. Sterilizationarrow_forwardMannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?arrow_forward
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