Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 17, Problem 5TYK
Summary Introduction
Introduction:
Transduction is the process by which foreign DNA (deoxyribonucleic acid) is introduced into a recipient bacterial cell by means of a virus or infecting phage (bacteriophage) or viral vector. The DNA containing the capacity of a phage is limited and only a small amount of donor’s DNA can be transferred to the recipient.
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When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :
A.
Ligation.
B.
Transformation.
C.
Transfection.
D.
Digestion.
Which of the following is defined as transformation?
A. Transfer of genetic information via a plasmid
B. Virus infects a host and deposits genetic material
C. Free floating DNA is uptaken
D. Transfer of genetic information from one bacterium to another
5.
Genetic information can be transferred horizontally from one bacterial cell to another cell of the "same generation" (versus daughter cells), leading to genetic recombination, by all of the following EXCEPT:
A. Gene expression (transcription/translation/protein synthesis)
B. Transformation
C. Conjugation
D. Transduction
E. None of the other four answers (all are mechanisms of “horizontal transfer” genetic recombination)
Chapter 17 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 17.1 - What are the properties of F+, F-, and Hfr cells...Ch. 17.1 - Prob. 2SBCh. 17.2 - Prob. 1SBCh. 17.2 - How does viral infection of an animal cell and a...Ch. 17.2 - Prob. 3SBCh. 17.3 - Prob. 1SBCh. 17 - Prob. 1TYKCh. 17 - Prob. 2TYKCh. 17 - Which of the following is not correct for...Ch. 17 - Prob. 4TYK
Ch. 17 - Prob. 5TYKCh. 17 - Prob. 6TYKCh. 17 - When a virus enters the lysogenic stage: a. the...Ch. 17 - An infectious material is isolated from a nerve...Ch. 17 - Prob. 9TYKCh. 17 - Prob. 10TYKCh. 17 - Prob. 11TYKCh. 17 - Discuss Concepts As a control for their...Ch. 17 - Prob. 13TYKCh. 17 - Prob. 14TYKCh. 17 - Prob. 15TYKCh. 17 - Prob. 1ITD
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A transducing particle injects all of the DNA that it contains into a bacterial cell. Which of the following are TRUE? A. The bacterium becomes Hfr B. The bacterium becomes F+ C. The bacterium immediately acquires new functions as the injected DNA is rapidly transcribed and translated into mRNA and proteins D. This injected DNA can integrate into the bacterium’s genome through a process called recombinationarrow_forwardIn Hershey and Chase’s experiment investigating which biological molecule carries genetic information, they found that phage DNA with radiolabeled phosphorus was retained in bacterial cells after transduction. To conclude that DNA is the genetic material, what assumption did they have to make? (photo linked) A. Protein does not enter the bacterium during transduction. B. DNA does not stay in the media after transduction. C. All of the phage DNA was radiolabeled. D. Bacteria can be transduced by phage.arrow_forwardUsing retroviral vectors for gene therapy might increase the patient's risk of developing cancer because they might: a. introduce proteins from the virusb. integrate recombinant DNA into the genome in ways that misregulate the expression of genes at or near the site of integrationc. not express the genes that were introduced into a patient's cellsd. not integrate their recombinant DNA into the patient's genomearrow_forward
- In nucleotide excision repair in E. coli, the function of the UvrA/UvrB complex is toa. detect DNA damage.b. make cuts on both sides of the damage.c. remove the damaged piece of DNA.d. replace the damaged DNA with undamaged DNA.arrow_forwardBacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infectionarrow_forwardA positive-strand RNA virus: a.must first be converted to a mRNA before viralproteins can be translated b.is not recognized by host ribosomes. c.can directly translate viral proteins. d.will be degraded by host enzymes. e.needs RNA dependent RNA polymerase to get converted into mRNAarrow_forward
- The function of CRISPR gene cluster in Streptococcus pyogenes is to Select one or more: a. destroy phage DNA in the bacteria. O b. protect phage DNA in the bacteria. Oc. repair the damaged DNA in its genome. O d. delete the specific genes in its genome.arrow_forwardQ: Antibiotics are used in genetic engineering. They are useful to keep culture free of microbial a O infections O to select healthy vectors .b O to identify replication start sites.c O as selectable markers .d Q: Choose the best choice that describes the purpose of adding chloramphenicol to the culture of engineered bacteria O It inhabits the process of protein a synthesis O It blocks chromosome replication .b It blocks cell division .c It leads to increase the copy number of d recombinant molecules Q: Alkaline denaturation process is a kind of DNA isolation on the basis of conformation, that cause insolubility of cell components except one of the following Supercoiled DNA a Chromosomal DNA b Protein molecules c RNA molecules d All choices.e None of choices f Q: After transforming the ligation mixture into the E. coli, the transformed and non- transformed bacteria both can grow on media .containing antibiotics True a O False ..barrow_forwardWhich statement(s) indicates one or more benefits of electroporation? A.) Possibility of introducing plasmids into cells B.) Very high transfection rate on neurons C.) Allows marking of endogenous proteins (i.e. naturally produced by the cell) with fluorescent proteins D.) Makes fluorescent cells without adding external productsarrow_forward
- Which of the following statements is not true? Select one: a. Viruses multiply inside living cells using viral - mRNA, tRNA & ribosomes. b. Viruses multiply inside living cells. c. Viruses contain DNA or RNA. d. The nucleic acid of a virus is surrounded by a protein coat. e. Viruses cause the synthesis of specialized infectious elements.arrow_forwardPlasmids are circular extrachromosomal DNA in bacteria that play a role in: d. degradation of viruses a. growth of bacteria b. pathogenicity and antibiotic resistance c. production of antibodiesarrow_forwardIn Hershey and Chase’s experiment investigating which biological molecule carries genetic information, they found that phage DNA with radiolabeled phosphorus was retained in bacterial cells after transduction. To conclude that DNA is the genetic material, what assumption did they have to make? A. Protein does not enter the bacterium during transduction. B. DNA does not stay in the media after transduction. C. All of the phage DNA was radiolabeled. D. Bacteria can be transduced by phage.arrow_forward
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