Concept explainers
You study a gene known to be important in the early stages of heart development. Loss of the gene is also suspected to play a role in triggering lung cancer. What kind of transgenic mouse would you use to study your gene in vivo?
a. Knockout
b. Knockin
c. Conditional knockout
d. All of the choices are correct.
Introduction:
Transgenic organisms contain a gene called transgene from different species, which is incorporated into their genetic composition through genetic engineering. The technique of genetic engineering also allows removal and change of gene as well.
Answer to Problem 1U
Correct answer:
Study on a gene that is known to be important in the early stages of heart development. Loss of the gene may trigger cancer. Conditional knockout is the kind of transgenic mouse that an individual would use to study that gene in vivo. Therefore, Option c. is correct.
Explanation of Solution
Reason for the correct statement:
Conditional gene knockout is a technique that is used to eliminate a specific gene present in the genome to study its effect on the development stages of the organism.
Option c. is given as “Conditional Knockout”.
As, “to study the effect of a gene that is known to be important in the early developmental stages of heart, conditional knockout transgenic mouse would be used” is the right answer.
Hence, Option c. is correct.
Reasons for the incorrect statements:
Option a. is given as “Knockout”.
Gene knockout is a technique that inactivates the gene, which results in loss of its functionality. Therefore, it is a wrong answer.
Option b. is given as “Knockin”.
Gene Knockin is a technique that includes replacement of the normal gene with the gene that has specific alterations. Therefore, it is a wrong answer.
Option d. is given as “All of the choices are correct”.
The study of the gene that has an important function whose loss leads to disease conditions can be done by a conditional knockout technique and not by knockout or knock-in technique. Since conditional knockout is the correct option, Option d is a wrong answer.
Hence, Options a., b., and d. are incorrect.
Conclusion:
Conditional knockout is a genetic engineering method that is used to eliminate a specific gene so that its effect can be studied.
Want to see more full solutions like this?
Chapter 17 Solutions
BIOLOGY VOL. II
- a) When does the process of X inactivation occur? 1. During the development of the egg 2. During embryo development 3. During fertilization 4. Immediately after birth b) Although most tortoiseshell cats are female, a litter of kittens will occasionally contain a male tortoiseshell. Which of the following can explain tortoiseshell coat color in a male kitten? 1. The male kitten has an extra Y chromosome 2. The male kitten has an extra X chromosome 3. The male kitten has no X chromosome 4. The male kitten cannot carry out X-inactivation c) How many Barr bodies will be in a body cell of a XXX (triple X) female? d) A female orange cat is crossed with an orange male cat. What are the phenotypes of the offspring by sex?arrow_forwardHow many Barr bodies will be in a body cell of an XY male?arrow_forwardNot use ai pleasearrow_forward
- a) After performing a reciprocal cross with a white-eyed female and a red-eyed male on a fruit fluy and then mate the F1 offspring to create an F2 generation: How did the results of the reciprocal cross differ from the first cross? b)To test whether the tan body trait (T) is sex-linked on a fruit fly: Describe anexperimental design and analysis. Is T a sex-linked trait?arrow_forwarda) The term “polylinker” in the pUC19 diagram is synonymous with what? b) Why should you never set your micropipette on the desk or otherwise turn it on its side? c) Describe the steps to use a 10 µL micropipe to draw and aliquot a 0.4 µL drop of water from a beaker of substrate into a microcentrifuge tube.arrow_forwarda) Describe two of the structural proteins in the SARS CoV2 virus and their purposes. b) Describe the coronavirus lifecycle.arrow_forward
- a) What does ELISA stand for and what is the purpose of this type of test. b) Which region of the antigen is at the tips, and what do the variable regions do during an infection to an antigen? c) What are the four components of the ELISA test and what do they do?arrow_forwarda) How does the Cas-9/RNA sequence target a piece of DNA for destruction? b) Why does DNA travel toward the positive electrode in the gel chamber? c) what is a ladder sequence and why is it useful to include in a gel?arrow_forwarda) What are the three steps of PCR, including temperatures, (or ranges?) b) What are two differences between next-gen sequencing and Sanger sequencing? c) What is a PAM sequence?arrow_forward
- We know that smoking tobacco cigarettes is associated with increased mortality, especially due to cardiovascular problems and cancers. Compare this situation to what we know (and don't know) about the dangers of marijuana smoke.arrow_forwardDiscuss one of the possible benefits of psychedelics on the treatment of mental and emotional conditions.arrow_forwardis medical care a public health issue? Should the government ensure that all people have access to the same quality of medical care? What are the arguments against this? What category of controversies may fit here?arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education