The application of FISH in obtaining the telomeric chromosomes in the G 2 phase of the cell cycle. Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

Do you expect any color or labeling in onion cells? Why not? 2. Would you expect Lugol's Solution to stain onion cells? Why or why not? Finally, look at cheek cells. Can you find the nucleus? 3. What strikes you as different in terms of cell shape compared to onion cells or cork cells? Why do you think that is? Think about differences between animal and plant cells (Figure 4.4 and text on page 59 of your textbook).

MICROGRAPH No. & Letter TYPICAL DRAWING Nucleus & chromatin: STRUCTURE 10 H Envelope & pores: Nucleus nuclear chromatin nucleolus envelope Nucleolus 10 C Nucleolus: pore Cytomembrane Rough ER: 1 A 6E (blow-up) Rough ER Ribosome: Ribosome 6 Folow-up) *Also locate ribosomes on rough ER in micro. 1 Smooth ER 9 J Smooth ER: Golgi: Golgi complex 7 B (six are shown in photo) LY90SOME 44505OME : Mitochondrion 4 B Mitochondrion:. 6 (blow-up) Chloroplast 9 C Chloroplast: Cytoskeletal Microfilaments 2 G Microfilaments: cross section (microfilaments inside a cut - lengthwise microvili) 2 H Microtubule 9N cross section lengthwise Microtubules: 11 D Intermediate (not visable in these no standard reprešentation filaments Intermediate filaments: micrographs) CILIA FLAGELLA Cell Structures and Adaptations - Worksheet

Tabulation of the differences between Eukaryotic and Prokaryotic Cells (Graphic Organizer) Name 10 parts of the cell and mark their differences: just mark (/) if present and (X) if absent. Example: Cell Eukaryotic Eukaryotic Prokaryotic Parts of the Plants Animal Bacteria cell 1. nucleus (/) (/) (X) 2.

Answer 2

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Answer 6

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Answer 7

Chapter 17, Problem 1DA

Summary Introduction

To determine: The application of FISH in obtaining the telomeric chromosomes in the G₂ phase of the cell cycle.

Introduction: Fluorescent in situ hybridization or FISH is the technique, which uses fluorescent probes or radioactive elements to diagnose and detect any chromosomal abnormality. For example, cancer cells are detected by this technique. Also, other abnormalities such as aneuploidy, deletions, and duplication are detected by FISH.

Answer 8

Expert Solution & Answer

Explanation of Solution

Humans are the eukaryotic diploid organisms, in which 23 pairs or 46 chromosomes are present. The telomeres are the nucleotide sequences present at each end of the chromosome and will produce 92 dots in fluorescent in situ hybridization. In the G2 phase of a cell cycle, the chromosomes prepare themselves for the division. Thus, the number of the chromosome will be doubled. Hence, the telomeres observed in the G2 phase will be increased in the number, that is, 184.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Ch. 17.1 - Prob. 1LO Ch. 17.1 - Prob. 2LO Ch. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LO Ch. 17.3 - Prob. 2LO Ch. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...

Concept explainers

Videos

Explanation of Solution

Want to see more full solutions like this?

Chapter 17 Solutions