Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Question
Chapter 16, Problem 16.14QAP
Interpretation Introduction
Interpretation:
The number of interferograms that have to be collected and averaged if goal is to obtain an
Concept introduction:
Interferometry refers to the technique of superposition of the
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A solution with a known concentration equal to 3.4 mM gave an absorbance reading of 0.820 in a spectrophotometer. Assuming that there is a linear relationship between concentration and absorbance up to a concentration of 4.0 mM, what would the absorbance reading be for a solution with a concentration 2.2 mM?
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The validation process comprises four main steps that are : hardware validation, software validation, method validation and system suitability. To evaluate a spectrophotometer’s accuracy by measuring absorbance, a solution of 60.06 mg/L K2Cr2O7 in 0.0050M H2SO4 was carefully prepared together with a 0.0050 M H2SO4 as a reagent blank. If the spectrometer’s signal is properly calibrated, it has been reported that this solution should provide an absorbance of 0.640 +- 0.010 at wavelength 350 nm. Which one of the validations mentioned above had been involved in this process? Explain your choice
Chapter 16 Solutions
Principles of Instrumental Analysis
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- What are the main components of a spectrophotometer? Choose all that applies. radiation source sample compartment/holder monochromator detectorarrow_forwardAtomic emission spectroscopy and the method of standard addition are used to determine the Na+ concentration and its uncertainty in an unknown sample. Increasing amounts of a 1.75 ug/mL standard Na+ solution are added to a series of volumetric flasks containing 15.00 mL of the unknown solution. The volumetric flasks are then diluted to a final volume of 200.0 mL. Atomic emission spectroscopy is then used to measure the emission intensity of each solution. Unknown volume Standard volume Final volume Emission (mL) (mL) (mL) intensity 15.00 0.00 200.0 351.0 15.00 5.00 200.0 494.0 15.00 10.00 200.0 642.0 15.00 15.00 200.0 807.0 15.00 20.00 200.0 948.0 Based on the data in the table, determine the original concentration of Nat and its uncertainty in the unknown sample. Report the uncertainty with three significant figures. concentration: ug/mL µg/mLarrow_forwardAnswer the questions regarding a single Fourier transform infrared spectroscopy (FTIR) experiment. The interferogram was sampled at a retardation interval (8) of 0.0001297 cm. What wavenumber range is possible with a sample retardation interval (8) of 0.0001297 cm? Aỹ = cm- For a spectrum acquired in 8-bit space (256 data points sampled) for ô = -A to d = +A , determine the value of maximum retardation (A) and the resolution (R). A = cm R = cm-1 What is the interferometer mirror velocity if each data point required 267 us (tintt) to obtain? Vint = cm/sarrow_forward
- 1. What is the concentration of the dye in a solution with a percent transmittance reading of 61.6% at 570 nm? 1a. If the solution in Part 1 was made by taking 10.00 mL of a stock solution and diluting it to 100.00 mL, what is the concentration of the dye in the stock solution?arrow_forwardWhat is the absorbance of a solution if it had 79.5% transmittance?arrow_forward
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