
Concept explainers
A.
To explain: What is observed in the budding of clathrin-coated vesicles from eukaryotic plasma membrane fragments when the adaptins are omitted.
Concept introduction:
Vesicular transport is needed to shuttle the proteins in and out of an organelle. Coated vesicles are the vesicles that bud from the membrane which have a unique protein coat on their cytosolic surface. The vesicles that have an outer coat made of protein clathrin are called a clathrin-coated vesicle. If the adaptins, clathrin, and dynamic-GTP are added to the membrane preparation, the budding of the clathrin-coated vesicle from the eukaryotic plasma membrane fragments can be seen.
B.
To explain: What would happen to budding clathrin-coated vesicles from eukaryotic plasma membrane fragments when the clathrin is omitted.
Concept introduction:
Vesicular transport is needed to shuttle the proteins in and out of an organelle. Coated vesicles are the vesicles that bud from the membrane which have a unique protein coat on their cytosolic surface. The vesicles that have an outer coat made of protein clathrin are called a clathrin-coated vesicle. If the adaptins, clathrin, and dynamic-GTP are added to the membrane preparation, the budding of the clathrin-coated vesicle from the eukaryotic plasma membrane fragments can be seen.
C.
To explain: What is observed in the budding of clathrin-coated vesicles from eukaryotic plasma membrane fragments when the dynamin are omitted.
Concept introduction:
Vesicular transport is needed to shuttle the proteins in and out of an organelle. Coated vesicles are the vesicles that bud from the membrane which have a unique protein coat on their cytosolic surface. The vesicles that have an outer coat made of protein clathrin are called a clathrin-coated vesicle. If the adaptins, clathrin, and dynamic-GTP are added to the membrane preparation, the budding of the clathrin-coated vesicle from the eukaryotic plasma membrane fragments can be seen.
D.
To explain: What is observed if the plasma membrane fragments were from a prokaryotic cell.
Concept introduction:
Vesicular transport is needed to shuttle the proteins in and out of an organelle. Coated vesicles are the vesicles that bud from the membrane which have a unique protein coat on their cytosolic surface. The vesicles that have an outer coat made of protein clathrin are called a clathrin-coated vesicle. If the adaptins, clathrin, and dynamic-GTP are added to the membrane preparation, the budding of the clathrin-coated vesicle from the eukaryotic plasma membrane fragments can be seen.

Want to see the full answer?
Check out a sample textbook solution
- 3) Which statement(s) about the Pacific Decadal Oscillation (PDO) is/are TRUE? CIRCLE ALL THAT APPLY. A. It is a major driver of salmon populations in the Pacific B. It affects sea surface temperatures in the eastern Pacific C. Its cycles typically do not last as long as those of ENSO D. Evidence that it has occurred over many centuries has been gathered from tree ring dataarrow_forward4.arrow_forward2arrow_forward
- 1. 2. 3. Marine fish cells are hypotonic compared to their seawater environment; their cells lose water by osmosis and gain solutes. If you add heterotrophic respiration and autotrophic respiration together and then subtract that value from gross primary productivity, then you have a more refined estimate of ecosystem carbon storage than NEE. Differential heating due to the earth's tilt generates the global wind AND oceanic circulation patternsarrow_forward1arrow_forwardCan you please simply explain what is going on here.arrow_forward
- Does it show the level of proteins? What about the amount? Levels of protein activation? How can you tell? Does the thickness tell you anything? What about the number of the lines? And the other questionsarrow_forwardKD 200- 116- 66- Vec ATF6 (670) ATF6 (402) ATF6 (373) ATF6 (366) I I 45- 1 2 3 4 5 ATFG (360) (e/c) 9V ATFG (402) g ant- ATF anti-KDEL DAPI barrow_forwardWestern blot results: what information can you get? Presence of proteins of your interest Levels of protein expression Levels of protein activation (must use activation state-specific antibody) Decreased function of the ATM kinase in aging mice. A C57BL/6 female 6 month Con IR 20 month C57BL/6 male 6 month 28 month Con IR Con IR Con IR p-ATM (S1981) ATM P-p53 (ser18) Actinarrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education





