Which type of nutrient medium would a double mutant with argG and argE grow on. Introduction: Beadle and Tatum selected Neurospora crassa , the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

In your laboratory, you have an F − strain of E. coli that is resistant to streptomycin and is unable to metabolize lactose, but it can metabolize glucose. Therefore, this strain can grow on media that contain glucose and streptomycin, but it cannot grow on media containing only lactose. A researcher has sent you two E. coli strains in two separate tubes. One strain, let’s call it strain A, has an F factor that carries the genes that are required for lactose metabolism. On its chromosome, it also has the genes that are required for glucose metabolism. However, it is sensitive to streptomycin. This strain can grow on media containing lactose or glucose, but it cannot grow if streptomycin is added to the media. The second strain, let’s call it strain B, is an F − strain. On its chromosome, it has thegenes that are required for lactose and glucose metabolism. StrainB is also sensitive to streptomycin. Unfortunately, when strains A and B were sent to you, the labels had fallen off the…

By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Analyze data. Compare and contrast. Make a drawing.

In E. coli, four Hfr strains donate the following markers,shown in the order donated:Strain 1: M Z X W CStrain 2: L A N C WStrain 3: A L B R UStrain 4: Z M U R BAll these Hfr strains are derived from the same F+ strain.What is the order of these markers on the circularchromosome of the original F+?

Answer 2

Question

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Answer 6

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Answer 7

Chapter 15, Problem 1DA

Summary Introduction

To determine: Which type of nutrient medium would a double mutant with argG and argE grow on.

Introduction: Beadle and Tatum selected Neurospora crassa, the bread mold for their experiments. They allowed the growth of cultures in a nutrient-rich medium and then subcultured individual fungal cells by placing them on minimal medium. This process enables the identification of cells that lost the capability to synthesize compounds essential for growth. They aimed to study the capability to synthesize arginine amino acid.

Answer 8

Expert Solution

Explanation of Solution

Beadle and Tatum selected mutants that can survive and grow on minimal medium with arginine. This resulted in a set of independent mutants that cannot synthesize arginine, and each of these mutants could be mapped genetically to different chromosomal positions. This research described four genes, namely argE, argF, argG, and argH. The specific lesion in each mutant can be determined by adding specific intermediates in the arginine biosynthesis pathway. Growth should be observed if the mutation influences the enzyme that occurs earlier in the biochemical pathway than the intermediate added to the minimal medium. No growth should be observed if the mutation influences an enzymatic step that occurs after the intermediate added.

The ArgE mutants are obstructed at the first step in the pathway. Therefore, the double mutant strain with argG and argE would grow only on nutrient medium supplemented with all the reaction intermediates.

Answer 9

Expert Solution

Answer 10

Expert Solution

Answer 11

Expert Solution

Answer 12

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.

Answer 13

Summary Introduction

To describe: What can a double mutant say about the order of the genes.

Answer 14

Expert Solution

Explanation of Solution

Generally, these types of double mutants enable researchers to conclude which gene exists first in the pathway. The double mutant will appear like an organism with a mutation at the initial point in the biological pathway.