BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)
10th Edition
ISBN: 9781305967359
Author: STARR
Publisher: CENGAGE L
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Textbook Question
Chapter 15, Problem 15SA
Match each term with the most suitable description.
| ___
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a.
|
| ___
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b. alleles commonly contain them |
| ___
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c. a person’s unique collection of short tandem repeats |
| ___
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d. requires reverse transcriptase |
| ___ transgenic | e. cuts
|
| ___ restriction enzyme | f. used in plant gene transfers |
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Match the method with the appropriate enzyme.___ PCR a. Taq polymerase___ cutting DNA b. DNA ligase___ cDNA synthesis c. reverse transcriptase___ DNA sequencing d. restriction enzyme___ pasting DNA e. DNA polymerase (not Taq)
FIGURE 2 shows the only suitable DNA restriction site in a
plasmid DNA vector that can be cleaved.
5'.GTAATCCGGATCCGAAATCCCCCGG... 3'
3.CATTAGGCCTAGGCTTTAGGGGGCCC... 5
FIGURE 2
Identify the most suitable enzymeto be used as a restriction enzyme forthis purpose.
O Ligase
O Smal
O EcoRI
О ЕсoRV
A recombinant DNA contains
-- and
Which of the following terms is appropriate for the blanks?
Lütfen birini seçin:
O a. gene to be cloned / cell
O b. plasmid / bacterial DNA
c. gene to be cloned/enzyme
O d. plasmid / gene to be cloned
O e. plasmid / protein
Chapter 15 Solutions
BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)
Ch. 15 - ________ cuts DNA molecules at specific sites. a....Ch. 15 - A _______ is a molecule that can be used to carry...Ch. 15 - Reverse transcriptase assembles an ________ on an...Ch. 15 - For each species, all ______ in the complete set...Ch. 15 - A set of cells that host various DNA fragments...Ch. 15 - Prob. 6SACh. 15 - Electrophoresis separates fragments of DNA...Ch. 15 - PCR can be used ______. a. as a cloning vector b....Ch. 15 - An individuals set of unique _______ can be used...Ch. 15 - Taq polymerase is used for PCR because it _______...
Ch. 15 - Put the following tasks in the order they would...Ch. 15 - True or false? Some transgenic organisms can pass...Ch. 15 - _______ can correct a genetic defect in an...Ch. 15 - True or false? Some humans are genetically...Ch. 15 - Match each term with the most suitable...Ch. 15 - Restriction enzymes in bacterial cytoplasm cut...Ch. 15 - The results of a paternity test are shown in the...
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- Bacterial ____ may be used as cloning vectors. a. polymerase b. endonuclease c. plasmids d. ligase e. transcriptasearrow_forward. 1 · | 2. L 3 | 4 | . 5 6 7 Direction: Encircle the letter of the correct answer . 1. It is a double-stranded , self replicating, circular DNA molecule present in bacteria which is widely used as a gene cloning vector. A. Cosmid B. Plasmid. C. Phagemid. D. Genome 2. In plant genetic engineering, which of the following acts as vector? B. Gene of interest D. Ti-plasmid А. Agrobacterium lumefaciens. Receipt plant cell. Which of the following illustrates breeding? A farmer choose a breed of cow for greater milk production The use of bacteria in order to produce human insulin. The insertion of cloned genes to plant cells. All of the above. 3. А. В. С. D. Being pest resistance is one of the traits that are being introduced to plants like corn and eggplant with the insertion of Bt-toxin gene to plant cell. What method is use when a gene is inserted to plants? A. Biolistic C. Plasmid insertion by heat shock treatment 4. B. Electroporation D. All of these 5. A biotechnologist wants to enhance…arrow_forwardDNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkagesarrow_forward
- Match each term with the most suitable description. ______ DNA profile a. GMO with a foreign gene ______ Ti plasmid b. alleles commonly have them ______ probes c. unique array of short tandem repeats ______ SNPs d. used to find clones. ______ transgenic e. mouse vs. human ______ genomics f. used in plant gene transfers ______ CRISPR g. based on RNA interferencearrow_forwardA plasmid is a circle of________ . a. RNA c. either RNA or DNA b. DNA d histone proteinsarrow_forwardMatch the terms appropriately.___ bacteriophage a. nitrogen-containing base,___ clone sugar, phosphate group(s)___ nucleotide b. copy of an organism___ diploid c. usually one-way___ DNA ligase d. injects DNA___ mutation e. seals gaps___ differentiation f. a pyrimidine___ cytosine g. can cause cancer___ semiconservative h. something old, something new replication i. two of each chromosomearrow_forward
- Definition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organismarrow_forwardPlasmid vectors have the following characteristics, except _________ . a. MCS b. expression c. transfection d. single stranded e. cloningarrow_forwardThe selection of the clones in plasmid-based directed mutagenesis depends on a. Replication error during the PCR O b. Reversing the resistance gene status O C. Blue colonies with disrupted LacZ O d. White colonies with disrupted LacZ O e. Plaques formation To overexpress a known gene of interest to produce high quantity of an industrial product you need to us a. Anti-sense RNA Ob. Anti-sense oligonucleotide O C. Ribozymes cloning Od. RNAi O e. Plasmid based directed mutagenesis. The CRISPR RNA in type II is composed of a. RNA sequence complementary to spacer DNA and CRISPR Ob. CRISPR RNA loop and spacer DNA O C. RNA sequence complementary to CRISPR DNA and spacer DNA O d. PAM sequence and Tracer RNA O e. Tracer RNA complementary to CRISPR RNA and the viral RNA The replication errors can be caused due toarrow_forward
- Use the set of gene sequencing results below to answer the question that follows: A G C T -Wells I 14. Based on the sequencing results above, what is the sequence of nucleotides as they were added by DNA polymerase to the DNA template? a/ATC-GCA-GTA b. TAG-TGC-CAT c. TAC-TGC-GAT GC-GA TAG-CGT-CAT L A EI la la اد ان YIarrow_forwardSmall circular molecules of DNA in bacteria ____. a. result from the activity of restriction enzymes b. are plasmids c. cannot survive outside of the cell d. are eventually degraded e. are DNA fragments from their main chromosomearrow_forwardDNA polymerase δ: DNA polymerase III as ________ a. Helicase: RPA b. DNA ligase: DNA polymerase I c. DNA polymerase alpha: primase d. DNA polymerase I: FEN-1/RNAse Harrow_forward
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