Explanation The reagent that serves as a mordant in the Gram stain procedure is iodine solution. After the smear is treated with this solution, it forms a complex with the primary stain crystal violet, which is trapped into the peptidoglycan layer of the bacterial cells...

LooseLeaf Benson's Microbiological Applications Laboratory Manual-Concise Version

14th Edition

ISBN: 9781259705236

Author: Alfred E Brown Ph.D., Heidi Smith

Publisher: McGraw-Hill Education

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Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.

Question

Chapter 14, Problem 5SAQ

Summary Introduction

To explain: The reagent that serves as a mordant in the Gram stain procedure and its function.

Introduction: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they contain the rigid cell wall. They are classified into different types based on the structure, namely coccus (spherical shaped), Bacillus (rod-shaped), filamentous, spirilla (spiral-shaped), appendaged, and pleomorphic bacteria. The structure of bacteria can be viewed under the microscope using the staining methods.

Expert Solution & Answer

Chapter 14 Solutions

LooseLeaf Benson's Microbiological Applications Laboratory Manual-Concise Version

Ch. 14 - Prob. 1SAQ Ch. 14 - How do gram-positive and gram-negative bacteria...Ch. 14 - How does the age of a culture affect the Gram...Ch. 14 - Which step in the Gram stain procedure is most...Ch. 14 - Prob. 5SAQ Ch. 14 - List the reagents of the Gram stain technique in...Ch. 14 - In what type of cell, gram-positive or...Ch. 14 - __________ Staphylococcus aureus before the...Ch. 14 - Prob. 2MQ Ch. 14 - Prob. 3MQ

Ch. 14 - Prob. 4MQ Ch. 14 - Prob. 5MQ Ch. 14 - Prob. 6MQ Ch. 14 - Prob. 7MQ Ch. 14 - Prob. 8MQ Ch. 14 - __________ Escherichia coli under the microscope...Ch. 14 - Prob. 10MQ

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