EBK BIOLOGY
11th Edition
ISBN: 8220101459800
Author: Byers
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 5MC
DNA cloning is
a. making multiple genetically identical cells.
b. making multiple copies of a piece of DNA.
c. inserting DNA into a cell.
d. changing the
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Researchers can isolate a sample of cells, such as skin fibroblasts, and grow them in the laboratory. This procedure is called a cell culture. A cell culture can be exposed to a sample of DNA. If the cells are treated with agents that make their membranes permeable to DNA, the cells may take up the DNA and incorporate it into their chromosomes. This process is called transformation or transfection. Scientists have transformed human skin fibroblasts with methylated DNA and then allowed the fibroblasts to divide for several cellular generations. The DNA in the daughter cells was then isolated, and the segment that corresponded to the transformed DNA was examined. This DNA segment in the daughter cells was also found to be methylated. However, if the original skin fibroblasts were transformed with unmethylated DNA, the DNA found in the daughter cells was also unmethylated. With regard to the transformed DNA, do fibroblasts perform de novo methylation, maintenance methylation, or both?…
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Chapter 14 Solutions
EBK BIOLOGY
Ch. 14 - Which of the following is not true of a single...Ch. 14 - Imagine you are looking at a DNA profile that...Ch. 14 - 3. Which of the following is not a commonly used...Ch. 14 - Prob. 4MCCh. 14 - 5. DNA cloning is
a. making multiple genetically...Ch. 14 - Are organisms that contain DNA that has been...Ch. 14 - 2._________ is the process whereby bacteria pick...Ch. 14 - 3. The______ is a technique for multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FTB
Ch. 14 - Prob. 1RQCh. 14 - 2. What is a plasmid? How are plasmids involved in...Ch. 14 - 3. What is a restriction enzyme? How can...Ch. 14 - 4. Describe the polymerase chain reaction.
Ch. 14 - What is a short tandem repeat? How are short...Ch. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - How are DNA probes used to identify specific...Ch. 14 - Prob. 8RQCh. 14 - Describe several uses of genetic engineering in...Ch. 14 - Prob. 10RQCh. 14 - 1. As you may know, many insects have evolved...Ch. 14 - 2. All children born with X-linked SCID are boys....
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- Why is the discovery of induced pluripotent stem cells important to the advancement of science and cloning? Select oner a. Can use in place of embryonic stem cells b. aids in nuclear transfer c. can use instead of red blood cells d. was the first successful cloning of a humanarrow_forwardA researcher wants to study cancer cells from a patient with breast cancer. Is cloning the cancer cells an option?arrow_forwardFor each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.arrow_forward
- Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forwardHow might people from different countries view the risks and benefits of biotechnology?arrow_forward
- Human growth hormone is being researched as a potential anti-aging drug. What is the most likely way that would scientists produce large amounts of growth hormone? O a. Use transcription factors to increase the rate of transcription of the relevant gene in humans Use the human growth hormone to develop an aging vaccine Use restriction enzymes to isolate the relevant gene and allow it to replicate in all types of cells O b. O c. O d. Transfer the relevant gene to a bacterial cell and culture large colonies then purify the product Oarrow_forwardIs cloning a form of genetic engineering? Explain your answerarrow_forward5) Below is an image that shows both reproductive and therapeutic cloning. Use this image to answer compare and contrast therapeutic and reproductive cloning. Are they used for similar means…etc. Once you have done that answer the question below. a) There are two types of therapeutic cloning. What are they and how are they different?arrow_forward
- What is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forwardTranscriptomes, that is, all the transcripts in a cell, are analyzed using: a. a type of PCR b. fluorescently labeled nucleotides c. microarrays d. all the abovearrow_forwardWhich of the following are strategies used by stem cells to reduce risk of alterations in their genomes and cancer (select all that apply)? A. Divide frequently so mutations during replication are diluted out B. Express high levels of xenobiotic transporters to pump exogenous chemical out of cells C. During replication, stem cells keep newly replicated strand while transit cells keep template copy D. Activate apoptosis pathways rather than DNA repair pathways when undergo DNA damage E. Located in tissue sites with minimal exposure to exogenous chemicalsarrow_forward
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