MICROBIOLOGY F/HEALTH SCIENCES
18th Edition
ISBN: 9781323737798
Author: CAPPUCCINO
Publisher: PEARSON
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Question
Chapter 13, Problem 58E.5RQ
Summary Introduction
To explain: The reason to add the restriction enzymes at last to the digestion mixture.
Introduction: The endonucleases are the enzymes, which are also called restriction enzymes or restriction endonucleases that are able to cleave the phosphodiester bonds of the DNA. These restriction endonucleases are able to cut the DNA at any specific ends. The use of restriction endonucleases has specific importance in the field of recombinant DNA technology.
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MICROBIOLOGY F/HEALTH SCIENCES
Ch. 13 - Prob. 1QCCh. 13 - Prob. 2QCCh. 13 - Prob. 53E.1RQCh. 13 - Explain what is meant by an operon.
Ch. 13 - Explain the purpose of the ONPG in the procedure.
Ch. 13 - Compare and contrast the methods for DNA transfer...Ch. 13 - Prob. 53E.5RQCh. 13 - Explain how genetic variations may be introduced...Ch. 13 - Prob. 54E.2RQCh. 13 - Distinguish between F+ and Hfr bacterial strains.
Ch. 13 - Explain the importance of the streptomycin marker...Ch. 13 - What mechanisms are responsible for antibiotic...Ch. 13 - Why is it necessary to use an antibiotic...Ch. 13 - Why has there been an increase in drug-resistant...Ch. 13 - Does the streptomycin in the medium cause the...Ch. 13 - Prob. 56E.1RQCh. 13 - What is the purpose of the biotin–histidine...Ch. 13 - What is the relationship between chemical...Ch. 13 - What are the advantages of using bacterial systems...Ch. 13 - Why is plasmid DNA preferred for genetic...Ch. 13 - What are selectable markers, and why are they...Ch. 13 - Prob. 57E.3RQCh. 13 - Alcohol is considered to be a significant reagent...Ch. 13 - Prob. 57E.5RQCh. 13 - Prob. 57E.6RQCh. 13 - Prob. 57E.7RQCh. 13 - Prob. 58E.1RQCh. 13 - Prob. 58E.2RQCh. 13 - Prob. 58E.3RQCh. 13 - Prob. 58E.4RQCh. 13 - Prob. 58E.5RQCh. 13 - Prob. 58E.6RQCh. 13 - Prob. 58E.7RQ
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