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Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
12th Edition
ISBN: 9780135188996
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 13, Problem 57E.3RQ
a.
Summary Introduction
To explain: The rationale for using EDTA in plasmid isolation.
Introduction: The plasmids are most commonly found in bacteria. The isolation of plasmid from the bacterial cells is a multistep process. The steps involved in the plasmid isolation are rupturing a bacterium that bears plasmid, removal of cellular components using various reagents, and finally, the plasmid DNA is suspended in an aqueous solution.
b.
Summary Introduction
To explain: The rationale for using sodium dodecyl sulfate (SDS) in plasmid isolation.
c.
Summary Introduction
To explain: The rationale for using potassium acetate in plasmid isolation.
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Chapter 13 Solutions
Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
Ch. 13 - Prob. 1QCCh. 13 - Prob. 2QCCh. 13 - Prob. 53E.1RQCh. 13 - Explain what is meant by an operon.
Ch. 13 - Explain the purpose of the ONPG in the procedure.
Ch. 13 - Compare and contrast the methods for DNA transfer...Ch. 13 - Prob. 53E.5RQCh. 13 - Explain how genetic variations may be introduced...Ch. 13 - Prob. 54E.2RQCh. 13 - Distinguish between F+ and Hfr bacterial strains.
Ch. 13 - Explain the importance of the streptomycin marker...Ch. 13 - What mechanisms are responsible for antibiotic...Ch. 13 - Why is it necessary to use an antibiotic...Ch. 13 - Why has there been an increase in drug-resistant...Ch. 13 - Does the streptomycin in the medium cause the...Ch. 13 - Prob. 56E.1RQCh. 13 - What is the purpose of the biotin–histidine...Ch. 13 - What is the relationship between chemical...Ch. 13 - What are the advantages of using bacterial systems...Ch. 13 - Why is plasmid DNA preferred for genetic...Ch. 13 - What are selectable markers, and why are they...Ch. 13 - Prob. 57E.3RQCh. 13 - Alcohol is considered to be a significant reagent...Ch. 13 - Prob. 57E.5RQCh. 13 - Prob. 57E.6RQCh. 13 - Prob. 57E.7RQCh. 13 - Prob. 58E.1RQCh. 13 - Prob. 58E.2RQCh. 13 - Prob. 58E.3RQCh. 13 - Prob. 58E.4RQCh. 13 - Prob. 58E.5RQCh. 13 - Prob. 58E.6RQCh. 13 - Prob. 58E.7RQ
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