Biology: Life on Earth with Physiology (11th Edition)
Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 12.2, Problem 1TC

Some viruses, such as the tobacco mosaic virus (TMV), consist of a protein coat surrounding ribonucleic acid (RNA) instead of DMA. A few years after the Hershey-Chase experiments, Heinz Fraenkel-Conrat and several colleagues separated TMV of two different strains (normal and HR) into their protein and RNA components. They then mixed the protein from strain HR with RNA from the normal strain, and vice versa. Hybrid viruses (either HR protein coats with normal RNA or normal protein coats with HR RNA) spontaneously assembled in these mixtures. They then allowed the hybrid viruses to infect tobacco plants and produce new viruses. If RNA is the genetic material of TMV, predict the type of protein coats formed by the offspring of hybrid viruses.

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In E. coli, the genes for histidine (his), arginine (arg), tyrosine (tyr), and valine (val) synthesis are closely linked. A  wild-type strain is used as a donor for transducing viruses. Various recipient strains were treated with these viruses. Wild-type recombinant colonies were then counted, with the results shown in the following table.   Genotype of recipient Number of wild-type colonies Genotype of recipient Number of wild-type colonies his-  arg+  val+ tyr+ 254 his+  arg-  val- tyr+ 132 his+  arg+  val- tyr- 36 his+  arg-  val+ tyr+ 240 his-  arg-  val+ tyr+ 28 his-  arg+  val- tyr+ 102 his-  arg+  val+  tyr- 100 his+ arg- val+ tyr- 2 What is the cotransduction coefficient of histidine and valine? For consistency, standardize on histidine.  0.38 (wrong)  0.537  0.402  None of these
The table below shows the properties of the genomes of three different viruses. The data were obtained as follows: Nuclease sensitivity was measured by the ability of deoxyribonuclease (DNase) or ribonuclease (RNase) to destroy the genome (a “+" means sensitivity). The ability of the genome to act as mRNA was tested by incubating it in a cell-free system. If amino acids were incorporated into protein, the data are shown as a Finally, the virus particles were tested for the presence of a virion polymerase. If an enzyme was present, the data show whether it could polymerize deoxynucleotide triphosphates (DNTPS) or nucleoside triphosphates (NTPS). "+. Genome Properties Nuclease Virion Can Genome Sensitivity? Polymerase? Be an mRNA? Virus DNase RNase With With DNTPS NTPS #1 - - #2 - - #3 For each virus, indicate the strategy of the genome, using the Baltimore classification. What is the nature of the product of the virion polymerase when present? + + + + + +
Two theoretical genetic strains of a virus (a-b-c- and a+b+c+) were used to simultaneously infect a culture of host bacteria. Of 10,000 plaques scored, the following genotypes were observed. Determine the genetic map of these three genes on the viral chromosome. Decide whether interference was positive or negative. a+ b+ c+ 4100 a- b+ c- 160 a- b- c- 3990 a+ b- c+ 140 a+ b- c- 740 a- b- c+ 90 a- b+ c+ 670 a+ b+ c- 110
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What Is A Virus ? ; Author: Peekaboo Kidz;https://www.youtube.com/watch?v=YS7vsBgWszI;License: Standard YouTube License, CC-BY