GENETIC ANALYSIS: AN INTEG. APP. W/MAS
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Chapter 11, Problem 1P
Summary Introduction

To analyze:

Question asked for the description on the structure of bacterial chromosome and plasmid with their properties.

Introduction:

Bacteria mostly contain single, circular chromosomes or linear chromosomes with double stranded DNA sequences. Bacterial genome is haploid in nature and ranges between 0.5 to 8 MB in size containing several thousand structural genes which code various proteins for their activities and metabolism.

Expert Solution & Answer
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Explanation of Solution

Escherichia coli and Bacillus subtilis are widely used bacterial species with single chromosome, among which E. coli is mostly used, and it has 4.6 million base pairs that encode about 4200 genes. In case of some bacteria, they show two or more chromosomes, e.g. Borrelia burdgorferi, Agrobacterium tumefacian, s and Methanococcus jannaschii. Some bacteria like Borrelia burdgorferi and Agrobacterium tumefacians shows linear chromosome.

Bacterial chromosome has little amount of repetitive DNA sequences in multiple copies at intergenic regions, though they rarely transcribe, they serve a key role in replication, recombination, or in regulation of transcription. In bacterial chromosome, chromosomes are densely packed in a series of tight loops in association with a number of nucleoid associated proteins (NAP), i.e. they show several loops by supercoiling of DNA. There are two types of associated proteins a) small nucleoid-associated proteins and b) structural maintenance of chromosomes proteins (SMC).

Conclusion

All aspects are determined.

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During the process of protein synthesis on a ribosome, when the large ribosomal subunit covalently attaches an amino acid to a growing polypeptide, what is the name of this newly formed covalent bond? the phosphodiester bond the peptide bond the aminoacyl bond the ether bond the glycosidic bond The ability of F+ cells, or Hfr cells, to transfer plasmid DNA to an F- cell is properly called: transversion transformation conjugation transduction transition
Assume that there are horizontal gene transfers between two completely different bacterial species. In one case it is a plasmid that is transmitted via conjugation, in the other case it is it is a part of the bacterial chromosome that is transferred via transformation. In which of the two cases is it likely that the transferred DNA will be present? left and can function in the recipient cells? Explain the biological background to your answer
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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license