Concept explainers
To analyze:
Question asked for the description on the structure of bacterial chromosome and plasmid with their properties.
Introduction:
Bacteria mostly contain single, circular chromosomes or linear chromosomes with double stranded DNA sequences. Bacterial genome is haploid in nature and ranges between
Explanation of Solution
Escherichia coli and Bacillus subtilis are widely used bacterial species with single chromosome, among which E. coli is mostly used, and it has
Bacterial chromosome has little amount of repetitive DNA sequences in multiple copies at intergenic regions, though they rarely transcribe, they serve a key role in replication, recombination, or in regulation of transcription. In bacterial chromosome, chromosomes are densely packed in a series of tight loops in association with a number of nucleoid associated proteins (NAP), i.e. they show several loops by supercoiling of DNA. There are two types of associated proteins a) small nucleoid-associated proteins and b) structural maintenance of chromosomes proteins (SMC).
All aspects are determined.
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Chapter 11 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
- The genes for both the α- and βglobin chains of hemoglobin contain introns (i.e., they are split genes). How would this fact affect your plans if you wanted to introduce the gene for α-globin into a bacterial plasmid and have the bacteria produce α-globin?arrow_forwardWhat color colonies will cells that contain a recombinant plasmid form?arrow_forwardWhat is the advantage for bacteria to have a circular DNA chromosome when eukaryotes have linear DNA chromosomes?arrow_forward
- List in the table below, the equivalent characteristics of the bacterial chromosomearrow_forwardFigure 3: Restrictrion site map showing the following: A) linear DNA that is not cut as reference B) DNA cut with BamHI, C) DNA cut with EcoRI, D) DNA cut with Hindlll 1. Calculate the size of the resulting fragments as they will occur after digestion and write the sizes on the maps below. Note that linear DNA has a total size of 48 502 bp A A DNA 10,000 20,000 30,000 40,000 48,502 (bp)arrow_forwardHigh Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into the cell 2) Genomic DNA incorporated into the cell 3) Viral DNA incorporated into the cell 4) Plasmid and genomic DNA from the donor cell is incorporated into the recipient cell's genome 5) The movement of genes within the cell through homologous recombinationarrow_forward
- The genome of D. melanogaster consists of approximately 1.7 * 108 base pairs. DNA synthesis occurs at a rate of 30 base pairs per second. In the early embryo, the entire genome is replicated in five minutes. How many bidirectional origins of synthesis are required to accomplish this feat?arrow_forwardHow many chromosomes are included in a typical bacterial genome?arrow_forwardDescribe the genetic makeup of a plasmid in an F’ cell, including the types and origins of genetic information contained in this plasmid, and the mechanism by which such a plasmid arises.arrow_forward
- a) Under normal conditions E. coli produces three DNA polymerases. State their functional similarities and differences. b) List the other proteins and enzymes involved in DNA replication in E.coli and give their functions.arrow_forwardLook at the double-stranded segment of DNA shown below. Imagine that the two strands have already been denatured, and the temperature has been decreased to an appropriate annealing temperature. Show where the two primers would anneal to the strands, then indicate the direction of extension on each new strand with an arrow. 5’--T C A G G A C G T A A G C T T G C A T A T C T C G A T G C T A A A T C A T—3’ 3’--A G T C C T G C A T T C G A A C G T A T A G A G C T A C G A T T T A G T A—5’ Primer #1: 3’ A C G A T T T 5’ Primer #2: 5’ G G A C G T A 3’arrow_forwardGive the complimentary DNA strand for the following:ACG TAG CTA GTC AGT CGT AGC Give the RNA strand for the following:ACG TAG CTA GTC AGT CGT AGC Using the provided amino acid table and the RNA strand you created in #2, create the amino acid sequence: Name and explain two different ways in which DNA can be damaged. Once DNA is damaged, can we repair it? If not, what are some possible outcomes from the damaged DNA?arrow_forward
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