NESTER'S MICROBIOLOGY
9th Edition
ISBN: 9781264826940
Author: Anderson
Publisher: MCG
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Textbook Question
Chapter 10, Problem 10MC
Which of the following is false?
a)Tropheryma whipplei could be identified before it had been grown in culture.
b) The GC content of DNA can be measured by determining the temperature at which double-stranded DNA melts.
c) Sequence differences between organisms can be used to assess their relatedness.
d) Based on DNA homology studies, members of the genus Shigella should be in the same species as Escherichia coli.
e) Gel electrophoresis is used to determine the serotype of an organism.
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You have been tasked with designing a new computer algorithm to identify protein-coding genes in bacterial genomes.a) Name 3 specific criteria/patterns you will use to define whether a given sequence could contain a full-length protein-coding gene, and briefly justify your choices.b) Do you think your algorithm will work well to detect proteins encoded by the archaean Sulfolobus acidocaldarius? Why or why not?c) Do you think your algorithm will work well to detect proteins encoded in the eukaryotic yeast Saccharomyces cerevisiae? Why or why not?
1. Which of the following bacterial species is not naturally competent?
A) Bacillus subtilis
В) Наеторhilus influenzae
C) Escherichia coli
D) Streptococcus рпеuтoniae
2. Bacterial species which are not naturally competent can be made competent in the laboratory by using
a combination of metal ions and temperature changes.
A) true
B) false
3. If 10 ng of plasmid DNA yielded 10,000 transformants per milliliter (mL), the transformation
efficiency is:
A) 105
В) 105
C) 107
D) 105
4. Under optimal conditions, all the bacterial cells in a sample can be transformed.
A) true
B) false
5. The plasmid called pGal, carries a gene encoding the enzyme beta-galactosidase. What is the natural
function of this enzyme?
A) It protects cells from a specific type of antibiotic.
B) It hydrolyzes the disaccharide lactose.
C) It is involved in plasmid replication.
D) It is necessary for transformation.
6. The pGal plasmid also carries a gene designated as amp". What is the function of this gene?
A) It protects…
1. What is a plasmid?
A) An organelle found in plants that conducts photosynthesis.
B) Circular extrachromosomal DNA.
C) An essential part of the bacterial chromosome.
2. What is the role of the NaOH in plasmid isolation?
A) It increases the pH.
B) It denatures the DNA.
C) Both of the above.
3. In which direction will the DNA migrate in an agarose gel?
A) To the positive pole.
B) To the negative pole.
C) That depends on the sequence of the DNA.
4. What is the function of SDS?
A) It denatures proteins.
B) It disintegrates the cell membrane.
C) Both of the above.
5. To which species group does E. coli belong to?
A) Bacteria,
B) Fungi.
C) Archaea.
Chapter 10 Solutions
NESTER'S MICROBIOLOGY
Ch. 10 - Name and describe the three areas of taxonomy.Ch. 10 -
2. Explain the basis for the three-domain systems...Ch. 10 -
3. Describe how a dichotomous key is used when...Ch. 10 - Why is MALDI-TOF MS particularly important in...Ch. 10 -
5. Describe the difference between using a probe...Ch. 10 -
6. Explain how signature sequences are used in...Ch. 10 - Describe the function of PulseNet.Ch. 10 - What is a phylogenetic tree?Ch. 10 - Prob. 9SACh. 10 - Describe how the GC content of DNA can be...
Ch. 10 - Which of the following is the newest taxonomic...Ch. 10 -
2. If an acid-fast bacterium is detected in a...Ch. 10 -
3. The “breath test” for Helicobacter pylori...Ch. 10 - The “O157:H7” of E. coli O157:H7 refers to the a)...Ch. 10 - When hydrogen peroxide is placed on a colony of an...Ch. 10 - Which of the following is best to use for...Ch. 10 - If the GC content of two organisms is 70%, the a)...Ch. 10 - If two microbial isolates have similar but...Ch. 10 - The sequence of which ribosomal genes are most...Ch. 10 - Which of the following is false? a)Tropheryma...Ch. 10 -
1. Microbiologists debate the use of biochemical...Ch. 10 - A researcher interested in investigating the...Ch. 10 - Prob. 1CTCh. 10 - When DNA probes are used to detect specific...
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