For a given solution, [ OH - ] pH , [ H 3 O + ] and whether the solution is acidic or basic has to be determined. Concept Introduction: pH: pH is a scale used to specify how acidic or basic a solution is. It ranges from 0-14. pH 7.0 is considered as neutral solution, pH more than 7.00 is taken as basic solution whereas pH less than 7.0 is considered as acidic solution (at 25 o C). It is the measurement of activity of free H + and OH - in solution. pH = − log [ H 3 O + ] pOH = − log [ OH − ] From ionization constant of water K w = [ H + ] [ OH - ] Ionization constant of water at 25 ο C K w = 1 × 10 − 14 . Less the pH , more is the acidity of the solution.
For a given solution, [ OH - ] pH , [ H 3 O + ] and whether the solution is acidic or basic has to be determined. Concept Introduction: pH: pH is a scale used to specify how acidic or basic a solution is. It ranges from 0-14. pH 7.0 is considered as neutral solution, pH more than 7.00 is taken as basic solution whereas pH less than 7.0 is considered as acidic solution (at 25 o C). It is the measurement of activity of free H + and OH - in solution. pH = − log [ H 3 O + ] pOH = − log [ OH − ] From ionization constant of water K w = [ H + ] [ OH - ] Ionization constant of water at 25 ο C K w = 1 × 10 − 14 . Less the pH , more is the acidity of the solution.
For a given solution, [OH-]pH, [H3O+] and whether the solution is acidic or basic has to be determined.
Concept Introduction:
pH:
pH is a scale used to specify how acidic or basic a solution is. It ranges from 0-14. pH 7.0 is considered as neutral solution, pH more than 7.00 is taken as basic solution whereas pH less than 7.0 is considered as acidic solution (at 25oC). It is the measurement of activity of free H+ and OH- in solution.
In this solution [OH-] is greater than [H3O+] and pH is more than 7.00. Hence this solution is a basic solution.
Hence this is a basic solution having pH=9.86 and [H3O+]=1.4×10-10M.
(b)
Interpretation Introduction
Interpretation:
For a given solution, [H3O+] the value of [OH-], pH and whether the solution is acid or basic that has to be determined.
Concept Introduction:
Concept Introduction:
pH:
pH is a scale used to specify how acidic or basic a solution is. It ranges from 0-14. pH 7.0 is considered as neutral solution, pH more than 7.00 is taken as basic solution whereas pH less than 7.0 is considered as acidic solution (at 25oC). It is the measurement of activity of free H+ and OH- in solution.
In this solution [H3O+] is greater than [OH-] and pH is less than 7.00 and hence the solution is acidic.
Hence this is an acidic solution having [OH-]=1.6×10-12M and pH=2.20.
(c)
Interpretation Introduction
Interpretation:
For a given pH, the value of the [H3O+] and [OH-] of the solution has to be calculated along with the prediction that whether the solution is acidic or basic.
Concept Introduction:
pH:
pH is a scale used to specify how acidic or basic a solution is. It ranges from 0-14. pH 7.0 is considered as neutral solution, pH more than 7.00 is taken as basic solution whereas pH less than 7.0 is considered as acidic solution (at 25oC). It is the measurement of activity of free H+ and OH- in solution.
As in this solution [H3O+] is greater than [OH-] and pH is less than 7.00 and hence the solution is acidic.
Hence this acidic solution have [OH-]=1.00×10-12M and [H3O+]=1.00×10−2M.
(d)
Interpretation Introduction
Interpretation:
For a given pH, the value of [OH-], [H3O+] and whether the solution is acid or basic that has to be determined.
Concept Introduction:
pH:
pH is a scale used to specify how acidic or basic a solution is. It ranges from 0-14. pH 7.0 is considered as neutral solution, pH more than 7.00 is taken as basic solution whereas pH less than 7.0 is considered as acidic solution (at 25oC). It is the measurement of activity of free H+ and OH- in solution.
Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL.
From a 2,000
μg/mL
BSA stock, create 1 mL of each of the following stock solutions in 0.02
M phosphate buffer using individual microcentrifuge tubes: 50
μg/mL,
250
μg/mL,
500
μg/mL,
1,000
μg/mL,
1,250
μg/mL,
1,500
μg/mL.
Be sure to properly label all the
microcentrifuge tubes before creating the standards.
You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a
powder agar, you will be preparing the desired concentration from a prepared stock
solution. Complete the information in the table and show your computation.
Volume of
Volume of diluent
Desired
Stock Solution
stock sol'n
needed to make
Concentration
needed
1000mL media
25%
12.5%
5%
1.5%
If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.
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