INSTRUMENTAL ANALYSIS-ACCESS >CUSTOM<
7th Edition
ISBN: 9781337783439
Author: Skoog
Publisher: CENGAGE C
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Chapter 1, Problem 1.7QAP
Interpretation Introduction
Interpretation:
The list of input transducer along with their use should be explained.
Concept introduction:
Transducers play a vital role in analysis process. The real physical world data can be converted into a measurable form with the help of these devices. Basically, transducer is a device which convert any form of signal into electrical signals or vice versa. They can either be used at input or output of a system. There are many factors which are considered while selecting a type of transducer to be used for a task. They are sensitivity, range, accuracy, precision, linearity, size and cost.
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Real life applications of standardization of NaOH.
The protein concentration of a protein isolate was determined using the Bradford assay. Five dilution of bovine serum albumin (BSA) were prepared from a 5.00 mg/mL
BSA stock solution. To these dilutions, 100 µl of Bradford reagent were added. After 5 minutes, the absorbance was taken at 595 nm.
Standard #
BSA,
in mg/mL
Volume of Bradford reagent, A595
in mL
1
5.0
0.000
1.00
5.0
0.134
3
2.00
5.0
0.265
4
3.00
5.0
0.388
4.00
5.0
0.497
The supernatant dilution was prepared by mixing 49 µL of the protein isolate with 16 µL water. The absorbance was taken 5 minutes after addition of 100 mL Bradford
reagent. Calculate the protein concentration (in mg/mL) of the original isolate if the absorbance at 595 nm was 0.413.
Note: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of calculation.
the ksp of baf2 is 2.45x10^-5
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