Biochemistry
8th Edition
ISBN: 9781464126109
Author: Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer
Publisher: W. H. Freeman
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Question
Chapter 1, Problem 16P
Interpretation Introduction
Interpretation:
The
Concept introduction:
The
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Peptides.
1. Draw the peptide Ala-Glu-Gly-Lys, as it would occur at physiological pH = 7.4.
The R groups of Ala and Gly are not acidic or basic, therefore do not have a pka and the charge
on these R groups is therefore independent of pH. Glu is acidic and Lys is basic, therefore the
charge on these amino acids is pH dependent. The pKas are shown below.
pKa
N-term = 9.0
C-term = 3.5
Glu4.1
Lys = 10.5
2. Draw a circle around the peptide bonds.
3. Label the C-terminus and the N-terminus.
4. What is the overall charge on the peptide at pH 7.4?
I. Buffer Preparation
A researcher in Biochemistry is isolating α-glucosidase enzyme from malted wheat flour. The protocol requires the use of 2.00L of 0.225 M of lactate buffer with pH of 4.25. How will he/she prepare this buffer from 2.00 M lactic acid solution and solid sodium lactate (NaC3H5O3) The Ka of lactic acid is 1.38 x 10-4. [ Na=23.0, C=12.0, H=1.01, O=16.0 g/mol]
Be sure to answer all parts.
Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems.
(K, = 5.01 x 109 and pk, 8.3)
%3D
%3D
(a) Calculate the pH of the Tris buffer after mixing 10.5 mL of 0.20 M HCI solution with 25.0 mL of
0.10 M Tris.
7.06
(b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00020
mol of H* was produced. What is the pH of the buffer at the end of the reaction?
(c) What would be the final pH if no buffer were present?
Chapter 1 Solutions
Biochemistry
Ch. 1 - Prob. 1PCh. 1 - Prob. 2PCh. 1 - Prob. 3PCh. 1 - Prob. 4PCh. 1 - Prob. 5PCh. 1 - Prob. 6PCh. 1 - Prob. 7PCh. 1 - Prob. 8PCh. 1 - Prob. 9PCh. 1 - Prob. 10P
Ch. 1 - Prob. 11PCh. 1 - Prob. 12PCh. 1 - Prob. 13PCh. 1 - Prob. 14PCh. 1 - Prob. 15PCh. 1 - Prob. 16PCh. 1 - Prob. 17PCh. 1 - Prob. 18PCh. 1 - Prob. 19PCh. 1 - Prob. 20PCh. 1 - Prob. 21PCh. 1 - Prob. 22PCh. 1 - Prob. 23PCh. 1 - Prob. 24PCh. 1 - Prob. 25PCh. 1 - Prob. 26PCh. 1 - Prob. 27PCh. 1 - Prob. 28PCh. 1 - Prob. 29P
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