You’ve collected a soil sample from an unexplored island and cultured a new bacterial species by growing it on complete media. Being a wild-type strain, you assume it is prototrophic. What would you do to determine whether this is true?
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You’ve collected a soil sample from an unexplored island and cultured a new bacterial species by growing it on complete media. Being a wild-type strain, you assume it is prototrophic. What would you do to determine whether this is true?
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- Your lab coordinator has decided to grow and maintain a sourdough starter. A sourdough starter is a live, on-going culture of yeast that is grown in flour, water, and a bit of sugar. It is used as an ingredient in some types of bread. This is the question : How does it accumulcate another species? You have been asked to take this mixed culture and isolate the different species in the starter (which is basically a liquid culture). How will you do it?When observing a streak plate of isolated bacteria as shown below, you see colonies outside the area you streaked. What would you concluded about these colonies?You've been assigned with isolating a certain species of bacteria from a mixed culture in order to characterize and identify the strain. Which technique(s) is/are the BEST to utilize? How will the procedure be carried out? Explain.
- For growth, a mutant bacterium requires that an amino acid not required by the wild parental strain be added to the growth medium. This mutant is known asThe process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteriaYou are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculated with your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made sure that all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage in the media. What is the most likely explanation? O You used the wrong bacteria or the wrong bacteriophage. There is an issue with the phage batch. O There is a problem with your bacterial culture of B. cereus. O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.
- Bacillus thuringiensis makes toxins that kill insects. These toxins must be applied several times during the growth season to prevent insect damage. As an alternative to repeated applications, one strategy is to apply bacteria directly to leaves.However,B. thuringiensis does not survive very long in thefield. Other bacteria, such as Pseudomonas syringae, do survive.Propose a way to alter P. syringae so it could be used as an insecticide.Discuss advantages and disadvantages of this approach compared with the repeated applications of the toxins from B. thuringiensis.Which among A-D is false regarding growth and/or analysis of a bacterial culture? A) O Fed-batch growth: typically results in an increased yield of cells over that obtained with batch growth B) O Auxotroph: requires addition of specific growth factors in order to grow C) O Prototroph: typically the wild type strain that possesses all the features indicative of the species D) O None of A-D is false; all are true statementsYou are studying a microorganism that contains a chloramphenicol acetyltransferase (CAT) enzyme, and looking for a clone of this microorganism that no longer contains the gene encoding CAT. Imagine you had an LB plate containing chloramphenicol. You streak an isolated colony of the Parent Strain and Clone A onto the same plate. Which of the following statements are true about the growth pattern after 24 hours in the incubator? Select all that apply? L-hlareeheticol LB chloremphenicel Parent Clune A Parent Clone A LD chlerephenicol 18 chloremphenitol Paremt Clone A Paront Clone A Plate A is the expected growth pattern as clone A should grow in the LB plate with chloramphenicol Plate B is the expected growth pattern as LB plate with chloramphenicol parent strain should grow in the Plate B is the expected growth pattern as clone A should not grow in the LB plate with chloramphenicol Plate A is the expected growth pattern as the parent strain should not grow in the LB plate with…
- You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?If you were to analyze three separate colonies from the plate above, would you expect them all to be the same species of bacteria? Why or why not?