You ran a gel using SDS-PAGE. Total molecular mass of your unknown protein (determined via gel filtration): 217 kDa Standard curve equation based on your protein ladder: 5205.6x^(-1.22) Number of bands in your unknown protein sample: 3 Distance each band traveled on your gel: A measured at 20 mm B measured at 62 mm C measured at 82 mm What is the subunit composition of this protein? 2 of A, 1 of B, 1 of C 1 of A, 2 of B, 1 of C 1 of A, 1 of B, 2 of C 1 of A, 1 of B, 1 of C

Q: Based on this table:  1) What is the difference between subunit mass and native mass?  2) How do…

A: The molecular weight and the isoelectric points are characteristics of the protein that help in the…

Q: Distinguish the two methodologies used for getting a complete protein structure. Will the structural…

A: Proteins are essential for every cellular process occurring in a biological system as they control…

Q: What is a Ramachandran Plot?

A: Ramachandran plot is developed by G N Ramachandran, C Ramakrishnan and V Sasisekharan in 1963. This…

Q: Subject: Biochemistry, chemistry, polyacrylamide gel electrophoresis Differentiate native PAGE and…

A: PAGE/SDS-PAGE are the electrophoretic techniques used for protein separation

Q: How could you improve upon your technique in order to get a better and more accurate standard curve…

A: A standard curve have the Absorbance values at a particular wavelength  (Absorbance at 595nm  i.e.…

Q: In the Bradford protein determination, the color of a dye changes from brown to blue when it binds…

A: Step 1:Beer's law equation is  A=ϵClA is absorbance, C is concentration.Now Absorbance is the…

Q: Some characteristics of three proteins are listed in the table below: Protein Molecular Weight…

A: Chromatography is a separation technique that separates a mixture of analytes based on their…

Q: How can I calculate the molecular weight of the unknown protein on this sample using the SDS-PAGE…

A: Electrophoresis of proteins is generally carried out in gels made up of the cross linked polymer…

Q: Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration…

A: Stock solutions are used in many laboratory procedures, in order to save space for keeping larger…

Q: Compute for Total protein concentration Given: Absorbance of standard = 0.280 Absorbance of sample =…

A: Total protein concentration= Asample/Astandard × Conc. of Standard For the above question the given…

Q: As an example, consider albumin, a protein made of a single polypeptide weighing 66,000 daltons (66…

A: Gel electrophoresis if the technique of separation of molecules on the basis of their size and…

Q: The graph below is a standard curve generated by plotting the distance travelled by the size…

A: The standard curve generated for an analysis involving separation of proteins along the SD-PAGE gel…

Q: How many copies of a protein need to be present in a cell in order for it to be visible as a band on…

A: * Given that you can load 100 µg of cell extract onto a gel and can detect 10 ng in a single band by…

Q: You are purifying a protein for the first me and decide to analyze it on SDS PAGE. You run two lanes…

A: A. This finding implies that the protein likely has disulfide links in its natural state. Disulfide…

Q: You ran a gel using SDS-PAGE. Total molecular mass of your unknown protein (determined via gel…

A: To determine the molecular weight of subunit C from the SDS-PAGE data, we'll use the provided…

Q: Consider a mixture comprised of the proteins below: Protein that will most strongly bind to an…

A: Chromatographic techniques are used to separate specific molecules from a mixture of different…

Q: a protein pretreated with an sds page detergent shows two bands around 100 and 150 kD on the…

A: SDS imparts an even charge and linearises your macromolecule. Beta-mercaptoethanol breaks down the…

Q: Predict the changes in specific activity (ex. increases, decreases) and concentration of your…

A: Protein purification is a set of procedures designed to separate one or more proteins from a complex…

Q: This is a picture of an SDS-PAGE gel. In the left lane are protein standards with molecular masses…

A: SDS-PAGE is used determining the molecular weight (MW) of an unknown protein. The first step the…

Q: Protein Description A 35 kDa monomer Disulfide-linked homodimer comprised of 19 kDa monomers…

A: Protein is made by amino acids and in living organisms, 22 types of amino acids exist and each has a…

Q: A C2 G This a photograph of an actual polyacrylamide gel that has been run with samples of protein.…

A: Note: Hi! Thank you for the question. We are authorized to answer three subparts at a time. Since…

Q: Several calibration curves were created for a series of protein standards of known molecular mass…

A: Introduction :- Chromatography is a laboratory method used in chemical analysis to separate a…

Q: Analysis of a protein is taking place. The enzymic acivity of this protein is stable up to…

A: Ion exchange chromatography is a type of chromatography where we separate the molecules based on…

Q: Look at the amino acid composition of a protein given in the table and click on the area in the 2D…

A: the isoelectric point of an amino acid is the pH at which the net electric charge of that amino acid…

Q: A protein is purified from a bacterium using Size Exclusion Chromatography (SEC), with a molecular…

A: It is a sieve tube chromatography technique, in which proteins are separated from other proteins…

Q: affinity

A:

Q: For the electrophoresis at 25°C of two proteins, in a medium with a viscosity of 0.001kg/m-s, the…

A: Electrophoresis is a technique used in biotechnology to separate and analyze biological molecules…

Q: Part C-Cold dentauration In the lecture, we observed that the protein stability curve as described…

A: Newton Raphson method is used to find solutions of any function. It is given by the…

Q: Mr standards without with (kDa) 2-МЕ 2-МЕ 200 100 90 75 50 25 20 15 | | |I| | |||

A: Proteins are polypeptide molecules made up of amino acids. Proteins are synthesized through the…

Q: Describe what these experiments tell you about the structure of the proteins.

A: Gel filtration chromatography- Components are separated based on their difference in molecular…

Q: Under the stated conditions, the majority of the protein molecules in solution would be

A:

Q: What kind of information is stored in protein three-dimensional structure. Why 3D structure…

A: Amino acids Proteins are the polymers of nitrogenous compounds called amino acids. Each amino acid…

Q: (a) 1 Normalized fluorescence 0.8 0.6 0.4 0.2 0 50 55 OM 0.100 M 0.200 M 0.300 M 0.500 M 1.00 M 2.00…

A: There are four classes of biological macromolecules - proteins, nucleic acid, lipids and…

Q: Can you help me understand how to measure both protein A and B please

A: PAGE is performed by loading a sample containing the molecules of interest onto the gel and…

Q: Examine this plot. Ligand X binding to Protein Y at different pH 0.9 0.8 pH 7 0.7 0.6 pH 5 0.5 0.4…

A: Proteins are specific to what they bind to. Anything that binds to a protein is termed as ligand.…

Q: A gel filtration column with a fractionation range of 1.5-20 kDa is used to separate out the…

A: Gel filtration chromatography is a technique that is used to separate molecules based on their…

Q: Does this calculation look correct? My goal isto have a target mass of 10 µg of my protein with a…

A: Yes this calculations look correct.since you want your final volume to be 30 microlitres you have to…

Q: Two proteins bind the same ligand, and the data is shown below. What is the KD value for Protein B?…

A: The answer of the above question is: Correct answer not given. KD is the equilibrium dissociation…

Q: Serum Albumin (Monomer) Ovalbumin 66,000 43,000 32mm 39mm 60mm 26mm 14,400 Lysozyme Unknown 1 ?…

A: Distance Molecular weight 23 132000   32 66000   39 43000   60 14400…

Q: 1.0 0.9 Z 0.8 15. A pharmaceutical company studied the binding of three different compounds, X, Y,…

A: Compound Z has the strongest affinity for the protein. This is indicated by the higher fractional…

Q: Four proteins Cytochrome C (pI=10.2) Myoglobin (pI=7.2), Hemoglobin (pI = 6.8) and Serum Albumin…

A: Electrophoresis is a process of separating the biological molecules such as DNA, RNA and protein on…

Q: The following data is protein melting point (Tm) characterization data obtained by the Zetasizer…

A: Proteins are biopolymers synthesized in the cells through translation. The proteins produced through…

Q: 5- a sample of a crude protein extract is obtained from a prostate cancer tissue read 0.98 at 280 nm…

A: According to Christian and Warburg method, if the sample have the *presence of nucleic acid also,…

Q: In the Bradford protein determination, the color of a dye changes from brown to blue when it binds…

A: Step 1:Let's go through this step-by-step to ensure we calculate the correct values for both parts…

Q: Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels. 1. A…

A: On basis of the gel used, electrophoresis is classified into 2 types, Agarose gel…

Q: 1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL…

A: The four types of biological macromolecules are nucleic acids proteins lipids and carbohydrates.…

Q: A solution containing two globular proteins each with a single subunit (one is "large" with a radius…

A: The amino acid links together to form a peptide, which forms the primary structure of the protein.…

Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.