You measure the absorbance of product as a function of time and get the following data (OD = optical density absorbance). time (s) Abs (OD) 0.120 4 0.151 0.182 12 0.213 Your enzyme factor is 28 uM/OD. What is the enzyme activity for this sample in units of uM/min? 0.465 2.6 0.017 13.02

The raw data is plotted in figure A. Then I transformed the absorbance data to make figures B and C.  A line (instead of a curve) on each plot would predict what reaction order for the rate law?

Pls help ASAP.

An enzyme-catalyzed reaction is studied in the absence and presence of an inhibitor and the following data was obtained.      [S] in mmoles/L Velocity in mmoles/L/min-1 No inhibitor With inhibitor 1.25 1.72 0.98 1.67 2.04 1.17 2.50 2.63 1.47 5.00 3.33 1.96 10.00 4.17 2.38               Calculate the Km of the enzyme in the reaction without inhibitor ________________________ Km’ of the enzyme in the reaction with inhibitor ________________________ Vmax of the uninhibited reaction ________________________ Vmax’ of the inhibited reaction ________________________

Kinetics question

A group of students compiled the data shown in data table 1 below.  What is the units for the average rate constant value (k)?   Exp. # [IO3-]0 (M)   [I-]0 (M)    [H+]0 (M) Time (s) 1 0.005 0.05 2 x 10-5 22.12 2 0.010 0.05 2 x 10-5 86.84 3 0.005 0.10 2 x 10-5 5.35 4 0.005 0.05 4 x 10-5 2.65     Question 8 options:   M-2 s-1   M-3 s-2   M2 s-2   M-4 s-2   M3 s3   M-4 s-3   M-2 s-2   M2 s2   M s   M s-1   M-3 s-1   M-1 s-1   M-3 s-3

Living chain polymerization of monomer X follows the first-order kinetics. The corresponding propagation rate constant (kp) was determined by gas chromatography (GC), with the following monomer concentrations measured at different polymerization times, assuming instant initiation: Polymerization time (min) 0 2 5 10 15 [X]t (M) 1.80 1.52 1.27 0.95 0.74 0.50 0.25 20 30 Plot the dependence of -In([X]/[X]) on time using software specialized for graphs (e.g., Origin, SigmaPlot, not MS Excell!) and perform the linear data fitting. Export the graph in TIFF format and paste it below as an image. Make sure the graph is graphically appealing! b) From this fitting, calculate the kp of polymerization, assuming the living character of polymerization and an initial initiator concentration (an thus the constant concentration of growing centers) of 0.09 M c) What is the theoretical degree of polymerization (DP) of the prepared polymer? We assume full monomer conversion, complete initiation, absence of…

For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration.  Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1.

The following initial rate data are for the reaction of hypochlorite ion with iodide ion in IM aqueous hydroxide solution: OCI+T> or + CI 1. Experiment [OCI] Rate 1 3.48x 103 5.05 x 103 1.01 x 10-2 5.05 x 103 1.01 x 102 1.34 x 10-3 2.68 x 103 2.68 x 10-3 5.36 x 103 2 3.48 x 103 6.97 x 10-3 6.97 x 103 4. a) What is the rate law equation? b) Determinek (give the numerical value). c) What is the rate of the above reaction when [OCI-]= 0.224 M and [I-]= 0.125 M?

The enzyme examase catalyses the following reaction, A - B where A is the reactant and B is the product A595 measurements were used to determine the catalytic activity of examase by measuring the rate at which B is produced. From this, the activity of the enzyme was determined to be 42 µmol/mL per minute. A standard albumin solution (concentration = 10 mg/mL) was used to prepare a standard curve of Abs595 vs amount of protein in mg. The protein assay was also performed using 100 uL of a 1 in 10 dilution of the original examase sample. For the absorbance measurements, it was determined that the sample used in the A595 measurement contains 1.2 mg of examase. Which of the following statements correctly gives the specific activity of examase? 1.0.35 U/mg O 2.0.035 U/mg 3. 5040 U/mg O 4.35 U/mg O 5.3.5 U/mg