For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration. Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1.

I am taking an online chem class and it’s harder than I thought. I have the answers to my whole study guide but I want to understand the WHY of the answers. Thank you!

2. The physical mechanism involved in elementary reactions was given as: A+ A k1 A* + A activation by binary collisions A* + A A + A deactivation by binary collisions k2 A* + bA *, products reaction step where b = 0 for unimolecular reaction steps b = 1 for bimolecular reaction steps b = 2 for termolecular reaction steps. Carry out a steady state analysis to show that unimolecular reactions are first order at high pressures, moving to second order at low pressures. State the rate determining steps in each region. In contrast, show that bimolecular reactions are second order at all pressures.

You have measured the absorbance of the enzyme-catalyzed reaction using alkaline phosphatase (E) and p-nitrophenol phosphate (S), then created the table below. What is the velocity of the Time (min) Absorbance (OD) 0.5 0.22 reaction? 1.0 0.63 1.5 1.1 2.0 1.5 .085 O 1.28 O 1.17 O .85