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You have isolated a temperature-sensitive cell-cycle mutant. The mutated gene encodes the activating subunit of the APC. The mutant protein is defective at high temperatures. You sort several thousand cells and recover the WT-looking profile below: Cells (in thousands) O G1 COM O m OS N Next you grow the mutant at the restrictive high temperature and sort an equal number of cells. First, what phase of the cell cycle do you expect the cells to arrest in? T 50 100 150 200 Propidium iodide fluorescence 250 #tv A @ X O

You have isolated a temperature-sensitive cell-cycle mutant. The mutated gene encodes the activating subunit of the APC. The mutant protein is defective at high temperatures. You sort several thousand cells and recover the WT-looking profile below: Cells (in thousands) O G1 COM O m OS N Next you grow the mutant at the restrictive high temperature and sort an equal number of cells. First, what phase of the cell cycle do you expect the cells to arrest in? T 50 100 150 200 Propidium iodide fluorescence 250 #tv A @ X O

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Draw and upload profile for the cells sorted at the restrictive temperature label the axes 

You have isolated a temperature-sensitive cell-cycle mutant. The mutated gene encodes the activating subunit of the APC. The mutant protein is defective at high temperatures. You sort several thousand cells and recover the WT-looking profile below: *Graph Explanation:* The graph is a plot of cell counts (in thousands) against propidium iodide fluorescence. The x-axis represents propidium iodide fluorescence, ranging from 50 to 250. The y-axis represents the number of cells, with values ranging from 0 to 5 (in thousands). The graph shows two peaks: a large peak at approximately 100 fluorescence units, and a smaller one near 200 fluorescence units. Next, you grow the mutant at the restrictive high temperature and sort an equal number of cells. First, what phase of the cell cycle do you expect the cells to arrest in? - ○ G1 - ○ M - ○ G2 - ○ S
Transcribed Image Text:You have isolated a temperature-sensitive cell-cycle mutant. The mutated gene encodes the activating subunit of the APC. The mutant protein is defective at high temperatures. You sort several thousand cells and recover the WT-looking profile below: *Graph Explanation:* The graph is a plot of cell counts (in thousands) against propidium iodide fluorescence. The x-axis represents propidium iodide fluorescence, ranging from 50 to 250. The y-axis represents the number of cells, with values ranging from 0 to 5 (in thousands). The graph shows two peaks: a large peak at approximately 100 fluorescence units, and a smaller one near 200 fluorescence units. Next, you grow the mutant at the restrictive high temperature and sort an equal number of cells. First, what phase of the cell cycle do you expect the cells to arrest in? - ○ G1 - ○ M - ○ G2 - ○ S
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