You have discovered a new species of plant. You isolate chromatin from the plant and examines it with the electron microscope and see characteristic beads on a string structure. After addition of a small amount of nuclease, which cleaves the string into individual beads that each contain 280 bp of DNA. With more digestion a 120-bp fragment of DNA remains attached to a core of histone protein. Analysis of the histone core reveals histones in the following proportions: H2A 33.3% H2B 33.3% H3 0% [no histone H3 found] H4 33.3% Based on these observations, what conclusions can you make about the probable structure of the nucleosome in the chromatin of this plant? Be specific in describing the nature of the nucleosome: which histones form the core (), how many of each are present () and size in bp of core-DNA that envelopes it ().
You have discovered a new species of plant. You isolate chromatin from the plant and examines it with the electron microscope and see characteristic beads on a string structure. After addition of a small amount of nuclease, which cleaves the string into individual beads that each contain 280 bp of DNA. With more digestion a 120-bp fragment of DNA remains attached to a core of histone protein. Analysis of the histone core reveals histones in the following proportions: H2A 33.3% H2B 33.3% H3 0% [no histone H3 found] H4 33.3% Based on these observations, what conclusions can you make about the probable structure of the nucleosome in the chromatin of this plant? Be specific in describing the nature of the nucleosome: which histones form the core (), how many of each are present () and size in bp of core-DNA that envelopes it ().
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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