You have already localized the genes to the same chromosome by deletion mapping, and now decide that the best way to accomplish the mapping is to conduct two simultaneous three-point testcross experiments. The genes you are investigating are as follows: N = round leaves, n = notched leaves; H = smooth stems, h = hairy stems; R = purple flowers, r = red flowers; B = grey seeds, b = black seeds; and Y = green pods, y = yellow pods. Earlier experiments you have done already established that gene B is in the middle of this gene cluster, so you design both three-point test crosses to include that gene. Cross #1 is designed as RrHhBb x rrhhbb while cross #2 is NnBbYy x nnbbyy. The results of both crosses are given in the table below. Based on the information given, determine the arrangement of these five genes including the position of each allele in the heterozygous fly and the distances between each pair of genes. (Hint: treat each experiment separately, knowing that gene B is in the middle!)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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You have already localized the genes to the same chromosome by deletion mapping, and now decide that the best way to accomplish the mapping is to conduct two simultaneous three-point testcross experiments. The genes you are investigating are as follows: N = round leaves, n = notched leaves; H = smooth stems, h = hairy stems; R = purple flowers, r = red flowers; B = grey seeds, b = black seeds; and Y = green pods, y = yellow pods. Earlier experiments you have done already established that gene B is in the middle of this gene cluster, so you design both three-point test crosses to include that gene. Cross #1 is designed as RrHhBb x rrhhbb while cross #2 is NnBbYy x nnbbyy. The results of both crosses are given in the table below.

Based on the information given, determine the arrangement of these five genes including the position of each allele in the heterozygous fly and the distances between each pair of genes. (Hint: treat each experiment separately, knowing that gene B is in the middle!)

 

b
см
см
cM
cM
Transcribed Image Text:b см см cM cM
Cross #1 Phenotypes
wild-type
Cross #2 Phenotypes
wild-type
number of offspring
number of offspring
84
23
red
64
notched
58
hairy
black
336
black
4
13
yellow
notched, black
notched, yellow
black, yellow
notched, black, yellow
413
red, hairy
red, black
16
423
344
2
hairy, black
hairy, black, red
58
57
85
20
Transcribed Image Text:Cross #1 Phenotypes wild-type Cross #2 Phenotypes wild-type number of offspring number of offspring 84 23 red 64 notched 58 hairy black 336 black 4 13 yellow notched, black notched, yellow black, yellow notched, black, yellow 413 red, hairy red, black 16 423 344 2 hairy, black hairy, black, red 58 57 85 20
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