
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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
Transcribed Image Text:You have a 25 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.93. You incubate
this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid
dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has
decreased to 6.15. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in
the original 25 mL sample. Remember to use significant figures in your answer.
сн,
CH,
сH, —с —о—сн, — сн, — N—сн,
H,0
но—сн, ——сн,— "N—сн, + сH,—ҫ—о + H
CH,
ČH,
Acetylcholine
Choline
Acetate
7.02
acetycholine in original sample:
nmol
Incorrect
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