You are responsible for the bioremediation of soil contaminated by jet fuel. Your coworker suggests you fertilize the soil with nitrogen and phosphorus to promote the growth of naturally occurring hydrocarbon-degrading microbes. However, you just read about a new mixture of microbes sold in a slurry with nutrients and a surfactant. What data do you need to collect to decide which approach would be best? How will you obtain these data?

You are responsible for the bioremediation of soil contaminated by jet fuel. Your coworker suggests you fertilize the soil with nitrogen and phosphorus to promote the growth of naturally occurring hydrocarbon-degrading microbes. However, you just read about a new mixture of microbes sold in a slurry with nutrients and a surfactant. What data do you need to collect to decide which approach would be best? How will you obtain these data?

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 2CR

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You're in charge of bioremediation of jet fuel-contaminated soil. Your colleague advises that you add nitrogen and phosphate to the soil to encourage the development of naturally existing hydrocarbon-degrading bacteria. However, you've just read about a new microbe slurry containing nutrients and a surfactant that's being marketed. What information do you need to gather in order to choose the best course of action? How are you going to get these numbers?
Assuming you are working in a microbiology laboratory and a client sent you a sample for the following analyses A)Identification of all microorganisms in the soil sample B) Enumeration of culturable bacteria in the sample C) Enumeration of both culturable and non-culturable bacteria in the sample For a to c, explain the following: Method of analyses to be used
You were tasked to aseptically collect different environmental samples from a quarry site and process them back in the laboratory for microbial cultivation and analysis. After all the thorough experiments, you were only able to cultivate and study 36 microorganisms. However, based on the metagenomic analysis conducted, there are about 140 other species present in the samples collected. Why is this the case? Discuss and expound on the possible reason
3. (a) Comparing the culture-dependant (e.g., colony forming units count) and DNA- based (e.g., PCR) bacterial number quantification methods, what are the primary disadvantages of using culture-dependent methods to quantify bacterial numbers in an environmental sample? Use no more than three sentences or bullet points to present your answer succinctly.
Discuss the possibility of life on Mars. In reference to extremophiles. Defend or refute this statement: The upper-temperature limit to life is unrelated to the stability of proteins or nucleic acids. How influential is microbial activity on the geological processes of mineral formation and deposition compared to biological processes? (argue both sides of this one). What challenges and advantages do you see with the use of extremeophiles in industrial or bioremediation applications?
As a technologist in the brewing industry, your job is to research and develop ways to improve the yield and quality of brewed beverages. You are responsible for growing and maintaining the particular strain of microorganism that is used in the fermentation of beverages. Name 2 environmental factors that would affect the growth of this organism and which you will need to optimize for abundant growth of your organism.
Microbes can provide beneficial activities. Bioremediation (using bacteria) is a process used to treat contaminated media, including water, soil and subsurface material". How?
Discuss the possibility of life on Mars. Will extremophiles prove useful? Can you explain the upper-temperature limit to life unrelated to the stability of proteins or nucleic acids? How influential is microbial activity on the geological processes of mineral formation and deposition compared to biological processes? Can you argue both sides? What are the challenges and advantages of the use of extremeophiles in industrial or bioremediation applications?
In microbiology, the term growth in a culture usually refers to an increase in O 1) the number and size of microbial cells. O 2) the amount of ATP consumed. O 3) a microbe's size. O 4) the number of microbial cells. 5) the amount of ATP produced.
Microorganisms are needed in wastewater treatment facility to do the actual breakdown and removal of nutrients and organic matter in the waste matter. One day, the employee who is in-charge of the facility notice that as the time goes by after the wastewater was loaded into the medium the metabolic activity of the microbe cease to increase and remain stable instead. Within the context of our discussion, what could be the possible explanation for the employee’s observation?
A soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.
A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?