You are presented with the following clinical scenario: "A 12 month old male child presents with recurrent bacterial infections. Flow cytometry shows a complete lack of B- lymphocytes." Using your knowledge of immunology and flow cytometry, identify which dot plots represent the healthy control and patient samples in each scenario and come up with a clinical diagnosis. Note that you may need to use additional background sources to fully understand what is going on at the cellular level for each diagnosis. What is the clinical diagnosis O HIV/AIDS O COVID-19 O LAD type 1 CD4-FITC CD8-PE CD45-PE CD8-PE 70% 13% 52% 10% 82% 27% 5% CD8-PE CD4-FITC CD20-FITC CD4-FITC 17% 38% 13% 68% Agammaglobulinaemia CD45-PE CD4-FITC CD45-PE CD45-PE 35% 8% 93% 22% CD15-FITC CD8-PE CD20-FITC CD15-FITC Select the flow cytometry dot plot that corresponds to the healthy control sample Select the flow cytometry dot plot that corresponds to the patient sample 30% 0% 77%

Diptheria, tetanus and pertussis (DTaP) vaccine: summery on this vaccine and make sure to include answer to these questions. Which pathogen does this vaccine protect against? Please list symptoms of infection, as well as the incidence of mortality or other complications, if this is known. What type of vaccine is this? i.e. Viral vector, live attenuated, subunit, toxoid, nucleic acid, etc. Does this vaccine contain an adjuvant? If so, please explain the type of adjuvant and which innate immune pathways it stimulates, if this is known. What is the dosing regimen, or schedule, of this vaccine? What is the efficacy of this vaccine, and how long can a patient expect to be protected post-immunization? Are there any scientifically confirmed side effects of this vaccine, and if so what is their rate of incidence? Thank you-

Match the following term with its description or definition. Immunoglobulin domain Amino acids that are separated in the protein chain but come together in the folded protein Antigen that carries several epitopes of the same or different specificity Heavy chain classes Soluble proteins that carry only one copy of the epitope ✓ Choose... IgG, IgM, IgD, IgA, IgE Multivalent antigen Discontinuous epitope Monovalent antigen Repeated barrel-shaped structure Choose...

1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge  true/false 3)  PBST  is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme  A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8