Write down the causes and solutions of the following problems encountered in DNA isolation. 1- low DNA concentration 2- Excessive DNA degradation 3- Obtaining low purity DNA

show the findings (DNA extraction-gel band; NO/YES) and briefly explain the interpretation of the results

PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?