Answered: With the aid of a diagram, explain the…

Science

Biochemistry

With the aid of a diagram, explain the principles of gel filtration chromatography.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Related questions

Q: Which of the alternatives below indicates techniques used both to purify and to concentrate…

A: Proteins are polymers of amino acids linked by peptide bond and it is one of the important…

Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…

A: The column chromatography procedure is used to separate a single chemical compound from a mixture.…

Q: Briefly explain that what is the important to use immobilization techniques in biosensor?...(explain…

A: Given: Immobilization techniques are the type of technical process in which enzymes are fixed to or…

Q: Give the principle involved in the ninhydrin test. What is its purpose?

A: The principle involved in the ninhydrin test is that the two molecules of ninhydrin react with the…

Q: Explain how gel filtration chromatography works. What type of gel will you used when the protein…

A: Chromatography is a process for separating components of a mixture based on their differential…

Q: Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS…

A: Electrophoresis is a method used to separate mixtures of RNA , DNA or proteins according to their…

Q: Define the what is gel electrophorsis.

A: Introduction Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that…

Q: Search for the principles and the uses of the following methods for protein measurements and provide…

A: In the question 5 different methods of protein measurement are given. Serum Protein Electrophoresis…

Q: How is it that smaller molecules move through a gel-filtration column more slowly than larger…

A: Various molecular and biological techniques are used today for the separation of materials in…

Q: What is the application of Gel Electrophoresis in healthcare? Cite at least 2 specific examples.

A: Gel Electrophoresis: A laboratory technique called gel electrophoresis is employed to divide…

Q: During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why…

A: For experiments involving growth of cells whether bacterial or eukaryotic the results of a growth…

Q: Why it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite…

A: Luminol is a chemical used in many laboratory experiments, like in biotech research lab, forensic…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate

A: The Agar plate is made from mixing agarose and a growth medium onto a petri dish. Agarose is in a…

Q: An iodine solution was sealed inside dialysis tubing, then immersed in a cup of water containing…

A: Dialysis membrane is a semipermeable membrane and allows only a few substances to pass on as…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: Since the development of the calcium chloride transformation procedure, other procedures have been…

A: Bacterial transformation is the transfer of DNA released from a donor bacterium into the…

Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…

A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: What are seven applications of gel filtration

A: Gel filtration is a biochemical technique by which molecules such as proteins or small molecules are…

Q: Explain about different types of bioreactors and how they are used in cell suspension culture. What…

A: Ans: Bioreactor It refers to any device that supports the biological environment. This is used for…

Q: Sample A: Sample B: Sample C: Sample D: Sample E: Sample F: 22mm 18mm 35mm 30mm 28mm 28mm 21mm 17mm…

A: Introduction The zone of inhibition is the area around an antibiotic pot where bacteria cannot grow.…

Q: Describe in detail the process of electrospray ionization ?

A: Ionisation is done by high voltage. Ions spread evenly on a surface. Ionised liquid gives rise to…

Q: Briefly explain how you could use the color of the Coomassie assay sample solution to determine if…

A: Colorimetric protein tests, for example, the Coomassie blue G-250 color (Bradford) examines, are…

Q: Which reagent removes contaminant proteins from DNA solution? Sodium dodecyl sulfate (SDS) O Sodium…

A: A major goal of the nucleic acid isolation is the removal of proteins . The seperation of nucleic…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: List three coating techniques used to make sol-gel coatings, and briefly describe each technique.

A: Sol-gel Is a method of creating solid materials from tiny molecules. The process involves the…

Q: In the assay of citric acid, what do you think about the nature of solution sample ? a) Before…

A: In the citric acid assay, the concentration of the citric acid is used to determine with the help of…

Q: How should you prepare your running buffer?

A: SDS-PAGE is method of separation of protein based on its size. Running buffer of the SDS-PAGE…

Q: Gel-filtration chromatography separates molecules according to their size . Smaller molecules…

A: Gel-filtration chromatography is a type of separation technique that uses a gel as a medium through…

Q: thanol has been carried over into your DNA sample (two answers possible here, select both of them)…

A: DNA is not soluble in ethanol . But when ethanol is there DNA gets separated by forming white…

Q: A microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its…

A: Psychrophilic bacteria are cold loving bacteria that grows and reproduce at lower temperatures…

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Question

100%

With the aid of a diagram, explain the principles of gel filtration chromatography.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Similar questions

List the following reagents in the order in which they are added to an ELISA: secondary antibody, bodily fluid sample, enzyme substrate, primary antibody O Scondary antibody, bodily fluid sample, primary antibody, enzyme substrate O Enzyme substrate, primary antibody, secondary antibody, bodily fluid sample Bodily fluid sample, enzyme substrate, secondary antibody, primary antibody Bodily fluid sample, primary antibody, secondary antibody, enzyme substrate
Biochemical tests such as the fermentation test can be used when unknown bacterial cells are identified. true or false?
Of the two principal polymers used in column chromatography and electrophoresis, which one would be most immuneto contamination by bacteria and other organisms?
Explain why it is essential that bacteria cultures be sterilized in an autoclave.
Porous dialysis tubing is used to create a "model cell" containing glucose and albumin, a protein. This "cell" is placed into a beaker containing water and Biuret reagent. Initially the "cell" is colorless and the beaker is light blue. At the end of the experiment, the "cell" is light purple and the beaker is still light blue. What accounts for the color change observed? O Biuret reagent entered the cell and reacted with protein. Glucose exited the cell and reacted with Biuret reagent. Biuret reagent entered the cell and reacted with glucose. O Protein exited the cell and reacted with Biuret reagent.
If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe growing within the zone of inhibitionfor a drug, what should her conclusion be?
briefly explain the principle behind this technique; gel-filtration chromatography
What is the principle of the gel electrophoresis separation technique?
A disk-diffusion test using E. coli gave the following results: Disinfectant Zone of inhibition (diameter in mm) A 15 B 2.0 C 10 D 5 In the table above, which compound was the most effective against E. coli? A) A B) B C) C D) D E) The answer cannot be determined based on the information provided.
Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved.
Describe how the following technique work on protein purification? ammonium sulfate precipitation
briefly explain the principle behind this technique; affinity chromatography using a diagram to illustrate.