Why should there be 3 banding techniques (G, Q, and R)? Aside from their differences in appearance and procedure, what is unique to each one of them? or what can one banding technique show that the others cannot?

Q: What is replica plating?

A:

Q: What are the official guidelines for coding and reporting? How are they used, and why are they…

A: The ICD-10-CM's formal conventions and instructions are accompanied by a collection of rules called…

Q: Given some strains of unknown genotype regarding thiamine and proline, how would you test their…

A: test for  the genotypes :

Q: Ethidium bromide used to be a common stain for detecting DNA/ RNA on agarose gel. (i) Describe how…

A: Ethidium Bromide is a DNA stain as well as a mutagen. This dye can be visualized under UV light. It…

Q: Why do we need compensation for multiple parameter fluorescence detection? And how does compensation…

A:

Q: When you run a sequencing reaction, you should include: a) only dideoxynucleoside triphosphates…

A: Ans- When you run a sequencing reaction, you should include a higher concentration of…

Q: one should run the following

A: Pairwise sequence alignment is one form of a sequence alignment technique, where we compare only two…

Q: In watermelons, bitter fruit (B) is dominant over sweet fruit (b), and yellow spots (S) are dominant…

A: Hi! Since you have posted a question with multiple sub-parts, we will solve the first sub-part for…

Q: Why do we need to amplify the DNA at all in order to visualize it in a gel?

A: PCR (polymerase chain reaction ) is a widely used technique to amplify DNA while Gel electrophoresis…

Q: Consider the following experiment. First, large populations of two mutant strains of Escherichia…

A: Mutation  The change in DNA sequence by either deletion, addition, duplication, transversion, or…

Q: Since higher concentration colcemid will result in shorter chromosome, you want to      change your…

A: It is given that: Molar concentration of the stock solution: 10 ug/ml.  Desired dilution…

Q: 1b) When doing automated sequencing, all 4 dideoxynucleotides are added to the same sequencing…

A: DNA sequencing is a biochemical method for determining the order of the nucleotide bases, adenine,…

Q: How does magnetic bead DNA extraction work?

A: DNA extraction is the method of isolating DNA by disrupting the cell wall and nuclear membrane with…

Q: What are the similarity and their differences between Benedict's and Fehling's tests?

A: Benedict's test and Fehling's test are used to determine the presence of sugars in solutions or…

Q: A very common molecular biology research method is to analyze cell or tissue homogenates by…

A: In molecular biology research, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting,…

Q: How is fluorescence in situ hybridization (FISH) used to producea spectral karyotype?

A: Fluorescence in situ hybridization (FISH) is a technique that is used to detect and locate a DNA…

Q: What is the structure of the gel stain being used? There are several other dyes that can bind DNA…

A: Gel staining is very important visualization and detection procedure that follows protein…

Q: Science 7-S1 - MI / The Basics of 5. Which of the following are functions of the nucleus? Select all…

A: INTRODUCTION Nucleus Nucleus is the membrane bounded organelle seen inside the cell that encloses…

Q: What variables can affect the transformation efficiency?

A: Introduction  The ability of cells to absorb extracellular DNA and express the genes that are…

Q: Ethidium bromide used to be a common stain for detecting DNA/ RNA on agarose gel. (i) Describc how…

A: During the extraction of DNA fragments via agarose gel electrophoresis, EtBr or ethidium bromide is…

Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?

A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…

Q: In an experiment assay, sometimes there are several dilutions involved. 0.5 mL of (Bovine serum…

A: Dilution is responsible for decreasing the solute concentration in the solution.

Q: Mingyu isolated double-stranded (ds) DNA from Matricaria chamomilla, a medicinal plant that is used…

A: Volume of ds-DNA = 2.50 mLVolume of distilled water = 12.00 mLActual concentration of ds DNA =…

Q: Horizontal sequence :VIRL Vertical sequence:MKF   Scoring rules: g/o = -3, g/e = -1, match or…

A: In bioinformatics, the Substitution matrix is a method of finding  the rate of similarity of a…

Q: Explain ORF analysis.

A: Protein translocation is a process where proteins move between cellular components. It can occur…

Q: What is the source of agarose, the powder used to make the gel?                        What is the…

A: Gel electrophoresis is a technique used by scientists to separate DNA or protein molecules.  Gel…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: I understand that microarrays are being used to define the molecular abnormality and the prognosis…

A: Leukemia and types

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: After incubation, the plates appeared as below: Water EvaGreen Ethidium Bromide SYBR Green 1

A: Ames assay is a method is used to determine whether a chemical can function as mutagen or not by…

Q: In the Hershey–Chase experiment, the radioactive label 32P was present inside bacterial cells (i.e.,…

A: DNA is an organic molecule that transmits information from generation to generation. Several…

Q: Considering the technologies that pave the way for the identification of molecules on the basis of…

A: Within the scope of recombinant DNA technology, the technologies that pave the way for the…

Q: Horizontal sequence :VIRL Vertical sequence:MKF   Scoring rules: g/o = -3, g/e = -1, match or…

A: A point accepted mutation (PAM) is the replacement of a single amino acid in the primary structure…

Q: What are dyes that stain DNA used for? Is background staining a problem when using such dyes?

A: The fragments of deoxyribonucleic acid (DNA) can be visualized by the dye after the separation…

Q: Several cell culture lines of epithelial cells, called “Cell Line A, B, or C,” are incubated with…

A: Epithelial cells are specialized cells that form the epithelium, a protective layer covering the…

Q: What are the 3 R's in animal experiments? Explain each.

A: The 3Rs principles were established more than 50 years ago to provide a foundation for conducting…

Q: Three pieces of cut DNA were isolated using gel electrophoresis.The DNA that is the longest will be…

A: At first, let's see what is gel electrophoresis? Actually, gel electrophoresis is a type of…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.