Why should there be 3 banding techniques (G, Q, and R)? Aside from their differences in appearance and procedure, what is unique to each one of them? or what can one banding technique show that the others cannot?

Q: What is replica plating?

A:

Q: What are the official guidelines for coding and reporting? How are they used, and why are they…

A: The ICD-10-CM's formal conventions and instructions are accompanied by a collection of rules called…

Q: Given some strains of unknown genotype regarding thiamine and proline, how would you test their…

A: test for  the genotypes :

Q: Ethidium bromide used to be a common stain for detecting DNA/ RNA on agarose gel. (i) Describe how…

A: Ethidium Bromide is a DNA stain as well as a mutagen. This dye can be visualized under UV light. It…

Q: Why do we need compensation for multiple parameter fluorescence detection? And how does compensation…

A:

Q: When you run a sequencing reaction, you should include: a) only dideoxynucleoside triphosphates…

A: Ans- When you run a sequencing reaction, you should include a higher concentration of…

Q: one should run the following

A: Pairwise sequence alignment is one form of a sequence alignment technique, where we compare only two…

Q: Why do we need to amplify the DNA at all in order to visualize it in a gel?

A: PCR (polymerase chain reaction ) is a widely used technique to amplify DNA while Gel electrophoresis…

Q: Consider the following experiment. First, large populations of two mutant strains of Escherichia…

A: Mutation  The change in DNA sequence by either deletion, addition, duplication, transversion, or…

Q: Below are 9 possible primer pairs. Determine which primer pair is the best choice by considering the…

A: Introduction :- Primers are short oligonucleotide sequence which are required for starting or…

Q: 1b) When doing automated sequencing, all 4 dideoxynucleotides are added to the same sequencing…

A: DNA sequencing is a biochemical method for determining the order of the nucleotide bases, adenine,…

Q: Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the…

A: Isoelectric focusing electrophoresis is a technique which is used for separation of amphoteric…

Q: How does magnetic bead DNA extraction work?

A: DNA extraction is the method of isolating DNA by disrupting the cell wall and nuclear membrane with…

Q: A very common molecular biology research method is to analyze cell or tissue homogenates by…

A: In molecular biology research, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting,…

Q: How is fluorescence in situ hybridization (FISH) used to producea spectral karyotype?

A: Fluorescence in situ hybridization (FISH) is a technique that is used to detect and locate a DNA…

Q: What is the structure of the gel stain being used? There are several other dyes that can bind DNA…

A: Gel staining is very important visualization and detection procedure that follows protein…

Q: What variables can affect the transformation efficiency?

A: Introduction  The ability of cells to absorb extracellular DNA and express the genes that are…

Q: Ethidium bromide used to be a common stain for detecting DNA/ RNA on agarose gel. (i) Describc how…

A: During the extraction of DNA fragments via agarose gel electrophoresis, EtBr or ethidium bromide is…

Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?

A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…

Q: Mingyu isolated double-stranded (ds) DNA from Matricaria chamomilla, a medicinal plant that is used…

A: Volume of ds-DNA = 2.50 mLVolume of distilled water = 12.00 mLActual concentration of ds DNA =…

Q: Horizontal sequence :VIRL Vertical sequence:MKF   Scoring rules: g/o = -3, g/e = -1, match or…

A: In bioinformatics, the Substitution matrix is a method of finding  the rate of similarity of a…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: What does SDS–PAGE stand for? What is the benefit of doing SDS– PAGE?

A: Electrophoresis is the migration of charged particles under the influence of an electric current.…

Q: What is the purpose of: A. The concentrated salt solution? B. adding isopropyl alcohol to the…

A: Introduction: DNA extraction is a process in which the DNA is separated from other cellular…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: In the Hershey–Chase experiment, the radioactive label 32P was present inside bacterial cells (i.e.,…

A: DNA is an organic molecule that transmits information from generation to generation. Several…

Q: Considering the technologies that pave the way for the identification of molecules on the basis of…

A: Within the scope of recombinant DNA technology, the technologies that pave the way for the…

Q: Horizontal sequence :VIRL Vertical sequence:MKF   Scoring rules: g/o = -3, g/e = -1, match or…

A: A point accepted mutation (PAM) is the replacement of a single amino acid in the primary structure…

Q: What are dyes that stain DNA used for? Is background staining a problem when using such dyes?

A: The fragments of deoxyribonucleic acid (DNA) can be visualized by the dye after the separation…

Q: It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit…

A: DNA is a double stranded helical molecule that is made up of repeating nucleotides. The nucleotides…

Q: Three pieces of cut DNA were isolated using gel electrophoresis.The DNA that is the longest will be…

A: At first, let's see what is gel electrophoresis? Actually, gel electrophoresis is a type of…

Q: Why was it necessary to mash the strawberries extensively with your hands? (hint: you wouldn’t have…

A: This is the process that is used for the extraction of DNA from the cell. DNA is present in the…

Q: What are streak patterns designed to do? What are the 4 steps in the quadrant streak method using a…

A: In microbiology, agar streaks plates are important as they allow bacteria and fungi to grow on a…

Q: In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to…

A: The absorption and subsequent re-radiation of light by organic and inorganic specimens is typically…

Q: The gels diagrammed below show the results of amplifying two VNTR loci from: 2 hairs found at a…

A: The individual contains two alleles for a gene in the genotype.

Q: In hybridizing microarrays, we use a solution that contains {3X SSC, 0.5% SDS, 5ug/ml salmon sperm…

A: The ratio of mass of solute present in a solution relative to the volume of the solution represents…

Q: In Figure 5-5,a. Why do A− and B− cells, by themselves, not formcolonies on the plating medium?b.…

A: A gene is the essential physical and functional unit of heredity. They are comprised of DNA…

Q: G CAT III || || || IIL| | III|| I | | II || | ||| || |L ||

A: Sanger sequencing, additionally called the “chain termination method”, maybe a technique for…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.