Why not just inject the double-stranded cDNA that codes for the spike protein of the virus? What additional step or steps would you need to use to get the human muscle cells to produce the spike protein if the cDNA was injected to serve as the virus?The new Covid19 vaccine produced by two companies (Pfizer, Moderna) uses mRNA coding for part of the spike protein of the virus. The virus uses the spike protein to invade human cells where it replicates. Is it surprising that the mRNA must be stabilized with chemicals that need ultra-cold or frozen storage to protect the mRNA from degradation before it causes human muscle cells to make the spike protein? (a) Lipid vesicleDNALipidbilayer(b) ElectroporationNe NElectricalpulse410(e) Biolistic deliveryDNA-coatedparticles(d) Microinjection(e) Virus infectionFigure 10-20Introduction to Genetic Analysis, Twelfth EditionO 2020 W. H. Freeman and Company

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Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter24: Viruses And Subviral Agents

Section: Chapter Questions

Problem 16TYU

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Why not just inject the double-stranded cDNA that codes for the spike protein of the virus? What additional step or steps would you need to use to get the human muscle cells to produce the spike protein if the cDNA was injected to serve as the virus?
The new Covid19 vaccine produced by two companies (Pfizer, Moderna) uses mRNA coding for part of the spike protein of the virus. The virus uses the spike protein to invade human cells where it replicates. Is it surprising that the mRNA must be stabilized with chemicals that need ultra-cold or frozen storage to protect the mRNA from degradation before it causes human muscle cells to make the spike protein?

(a) Lipid vesicle
DNA
Lipid
bilayer
(b) Electroporation
Ne N
Electrical
pulse
410
(e) Biolistic delivery
DNA-coated
particles
(d) Microinjection
(e) Virus infection
Figure 10-20
Introduction to Genetic Analysis, Twelfth Edition
O 2020 W. H. Freeman and Company

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