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Biochemistry

why is the maximum velocity in the michaeli's equation not touching the rectangular hyperbolic curve

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter3: Biosignals And Noise

Section: Chapter Questions

Problem 4P

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Write C if only statement A is correct, H if only statement B is correct, E if both statements are correct, M if both statements are incorrect. (A) For the Lineweaver-Burk plot, the slope of the line reflects the ratio between the maximum velocity and the Michaelis constant. (B) Meanwhile, the y-intercept is the maximum velocity.
You have measured the following data for enzyme X. Substrate Vo concentration (micromolar (mM) Isec) 0.05 10 0.1 60 0.5 175 1 250 350 490 10 492 20 494 50 499 100 498 200 498
The graph of the Michaelis-menten equation, vo against [So] is unsatisfactory as a means of determining Vmax and Km. Briefly discuss why this is the case. How did lineweaver and buck overcome this problem?
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Using the Michaelis-Mentan graph pictured (graphing initial velocity and PNPP concentration), identify Vmax and Km as well as possible. Then, using the Lineweaver-Burk graph (graphing 1/Vo and 1 / PNPP concentration), label the reciprocals of Vmax and Km on the graph and use them to calculate Km and Vmax.
Using the above “mean-speed theorem”, calculate the average velocity of a car with constant acceleration from 0 km/hour (initial speed) to 80 km/hour (final speed) over a 5-minute period of time. average velocity over 5 minutes = 16 kilometers/hour average velocity over 5 minutes = 20 kilometers/hour average velocity over 5 minutes = 24 kilometers/hour average velocity over 5 minutes = 32 kilometers/hour average velocity over 5 minutes = 40 kilometers/hour
Kepler’s third law can be stated mathematically as: velocity = distance/time (years)2 = (A.U.)3 S = vot + ½ (a) t2 vm = ½ (vo + vf) F = GM1M2/d2
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Based on the speculations of Nicole Oresme, and on the equation relating spatial distance, time, initial velocity, and constant acceleration developed by the Mertonian Calculators, how far would you expect a falling object to travel in 4 seconds (falling from a spaceship towards the Earth, in the vacuum of space), starting at 0 feet/second, with constant acceleration (32 feet/sec2), and neglecting possible air friction? 64 feet 144 feet 256 feet 400 feet 576 feet
Can you please calculate the following?
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An airplane flying directly eastward at a constant speed travels 293 km in 2.0 h. (a) what is the average velocity of the plane? (b) what is the instantaneous velocity?