Why is it important to see the level of solvent in the developing chamber when running a TLC experiment.?

What is the purpose of thin layer chromography lab? What is a hypothesis I can use when conducting a TLC lab and how can that hypothesis be tested?

5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.

Based on TLC, how can you tell if the reaction is complete and what is the relevvance of the multiple marks in the lanes of the tlc? PROCEDURE To a six inch test tube was added thiamine hydrochloride (0.65 g), followed by water (2mL). The mixture was gently shaken until homogeneous, and then ethanol (7.5 mL)was added. Finally, sodium hydroxide (10%, 1.5 mL) was added and the mixture wasgently stirred to ensure complete mixing.Benzaldehyde (3.8 mL) was then added at room temperature in one portion with gentlestirring. The pH of the resulting reaction mixture was determined and adjusted to >10by dropwise addition of 10% sodium hydroxide. The reaction mixture was then partiallysubmerged in a 65 °C water bath2 and the progress of the reaction was periodicallymonitored by TLC analysis.Once TLC analysis indicated that the reaction was complete, the reaction mixture wascooled to approximately room temperature, and then chilled in an ice-water bath. Theresulting precipitate was collected by…

Suppose triphenylmethanol and biphenyl have R¢ values of 0.30 and0.60, respectively, in a certain TLC experiment. Sketch the final appearance of a TLC plate, roughly to scale, including the starting line and solvent front a. for a pure triphenylmethanol sample b. for a triphenylmethanol sample contaminated with biphenyl

3RD