If a TLC was run in a solvent system that caused all the spots to show up at the baseline, how could the system be adjusted so that Rf values could be determined?

What is the purpose of thin layer chromography lab? What is a hypothesis I can use when conducting a TLC lab and how can that hypothesis be tested?

5. Properties and IMF: Chlorophyll a and Chlorophyll b are almost identical in structure, but they separated during TLC. Report the RFs you recorded for both molecules. What functional group(s) are different between them? Then explain these RF values by comparing the IMF present in each molecule to those present in the mobile or stationary phase and how this shaped their affinities for each phase. Use the terms RF, IMF, polar, nonpolar, affinity, mobile phase and stationary phase.

5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.

does a co spot on TLC plate have two Rf values?

Organic Chemistry, Column questions

If the stationary phase and the compound being tested are both polar, will the retention factor (Rf) likely be smaller or larger than if a nonpolar compound was tested? Why? 1) The retention factor will likely be smaller as it has a smaller interaction with the stationary phase O 2) The retention factor will likely be greater as it has a smaller interaction with the stationary phase O3) The retention factor will likely be smaller as it has a greater interaction with the stationary phase 4) The retention factor will likely be greater as it has a greater interaction with the stationary phase

The TLC plate shown was run in 80:20 Petroleum Ether - Ethyl acetate. If the eluent is changed to 95:5 Petroleum Ether - Ethyl Acetate, what will happen to the R-values? All Rf values will decrease All Rf values will increase Some Rf values will increase Some Rf values will decrease