Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a "needle" used to inoculate?
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Hydrogen Sulfide Production
A growth medium is a also known as a culture medium which can be solid, liquid, and even semi-solid. It is prepared in such a way it provides a appropriate conditions for the proliferation of microbes, or even tissues of plants and animals in vitro. Based on the type of cells growing and its requirements, the media has various components to sustain the growth of the culture.
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- Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a “needle” used to inoculate?Is the Mueller-Hinton Agar (MHA) a complex or defined medium? Explain based on its composition. Is MHA a A) differential, B) selective, C) both differential and selective media, or D) neither? Explain based on what kind of microorganisms it allows to grow.Not only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate? Multiple Choice a) Gamma-hemolytic b) Alpha-hemolytic c) Non-hemolytic d) Beta-hemolytic
- The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilleIf the pH indicator were left out of MacConkey agar, the medium would bea) complex.b) differential.c) defined.d) defined and differential.e) complex and differential.A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?
- You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) FalseWrite a paragraph explaining the Gram stain result and the differences in the endospores of these three spices: a) Clostridium perfringes b) Clostridium tetani C) Clostridium botulinum1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilled
- A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?six Staphylococcus aureus are inoculated into a cream pie by the hands of a pastry chef. The generation time of S. aureus in cream pie at room temperature is 30 minutes. a) How many S. aureus are in the pie after 4 hours at RT? b) After 24 hours?N Please observe the following cells that have been Gram stained. "* 3 E ER $ JO 4 R % 5 6 F G & Y I U 8 00