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Why do we interpret positive hemolysis in the complement fixation
test to mean negative for the test substance?
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- How is a direct agglutination test different from a passive agglutination test?Give the full form of ELISA.which disease can be detected using it?Discuss the principle underlying the test.A nine-laboratory cooperative study was performed to evaluate quality control for susceptibility tests with 30-µg netilmicin disks [6]. Each laboratory tested three standard control strains on a different lot of Mueller-Hinton agar, with 150 tests performed per laboratory. For protocol control, each laboratory also performed 15 additional tests on eachof the control strains using the same lot of Mueller-Hinton agar across laboratories. The mean zone diameters for each of the nine laboratories are given in Table 6.11. *6.20 Provide a point and interval estimate (95% CI) for the interlaboratory standard deviation of mean zone diameters for each type of control strain, assuming each laboratory uses different media to perform the susceptibility tests. *6.21 Answer Problem 6.20 assuming each laboratory uses a common medium to perform the susceptibility tests. 6.22 Are there any advantages to using a common medium versus using different media for performing the susceptibility tests with…
- Is the RPR Test specific for Syphilis? Why or why not?Serial dilutions are made in order to determine the level of the antibody in the sample. True or False ?You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flask
- 7 mL of a 1:50 dilution is needed to run a specific serological test. How much serum and how much diluent are needed to make this dilution? A 1:5 dilution of patient serum is necessary to run a serological test. There is 0.2 mL of serum that can be used. What amount of diluent is necessary to make this dilution using all of the serum?An ELISA was performed on the sera of three patients to detect antibody against varicella (the chickenpox). Give the titer results shown in the answers, which patient's serum had the most anti-varicella antibody? There is not enough information to answer the question. Patient 2, dilution 1:30 O Patient 1, dilution 1:2,000 O Patient 2, dilution 1:400What is the recommended method to identify an antibody causing a suspected ABO based HDN( Hemolytic Disease of newborn)?