Why are some of the detectors listed unsuitable for HPLC?

Q: Identify some advantages and limitations of using HPLC, and explain

A: When organic mixtures of compounds are nonvolatile, thermally not stable, and have high molecular…

Q: Describe the sample properties that would be suitable for; i) Gas chromatography (GC) analysis ii)…

A: GS and HPLC are the techniques to separate compounds from mixtures .These are nalytical methods of…

Q: All chromatographic techniques can be used as separation methods.  True  False

A: Chromatography is a laboratory technique for the separation of a mixture into its components.…

Q: VGR 9. This is the TLC plate for the solvent: Ethyl acetate 50% / acetone 50% V is crystal violet, G…

A: Retention factor (Rf) is the distance travelled by the compound divided by distance travelled by the…

Q: How the 2D Thin layer Chromatography separation processes are worked? Please shortly wtite at your…

A: A 2D - TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid…

Q: The retention factor for analytes in a sample lie between 1 and 5 why?

A: The analyte is the substance to be separated during chromatography. It is also normally what is…

Q: How to improve the sensitivity and selectivity of AFS for Hg detection? Please explain.

A: If we increase the intensity of excitation source in AFS ,  high sensitivity will be…

Q: How can you vary the retention factor and selectivity factor?

A: Retention factor is a variable which indicates the time duration a component spends in the…

Q: Can an HPLC run be stopped during operation without impairing the separation

A: HPLC- High performance liquid chromatoghraphy It consists of two phase .One is stationary phase and…

Q: It is not of the solute property detector used in HPLC is: a. Refractive index detector b. UV…

A: Mixture components are estimated using HPLC detectors. The actual separation in HPLC (High…

Q: Calculate: Retention factor (k), Separation factor (α), and Resolution (R) for slide 29. Comment on…

A: Retention factor in GC =(tr-t0)/t0 is ratio of  analyte amount in stationary phase to mobile phase.…

Q: .Which of the following solvents is not commonly used in reversed-phase HPLC? a) Ethanol Water b)…

A: The solvent which is not used commonly in HPLC has to be given,

Q: 1. Define sparging. What is the significance of this step in an HPLC analysis?

A:

Q: wo components in an HPLC separation have retention times that differ by 22 s. The first peak elutes…

A:

Q: density

A: According to the question, the readings are: 0.24, 0.35, 0.67, 0.89. We need to determine the…

Q: 2. a) What is a bonded phase in HPLC? b) Give an example of a bonded phase in reversed-phase liquid…

A: Bonded Phase is the compound that is directly attached to the stationary phase in the liquid…

Q: What are the advantages of using a multidimensional HPLC separation for the analysis of complex…

A: Since you have asked multiple questions, we will solve the first one for you. For remaining…

Q: A chromatography column would be most efficient with which of the plate heights? 0.50 mm 0.10 mm O…

A: Correct answer is option B i.e 0.10 mm

Q: 10. which of the followings is the universal detector used in IHPLC: a) Fluorescence b) IR c) FID d)…

A: HPLC ("High-Performance Liquid Column") is utilized to separate and distinguish the components that…

Q: What is Ultra-Performance Liquid Chromatography (UPLC)? What are the differences and advantages of…

A: The newest technology in liquid chromatography-based analysis is Ultra Performance Liquid…

Q: Explain why a chromatographic separation normally has an optimum flow rate that gives the best…

A: When an fast flow rate is maintained, the band is not allowed to spend much time within the column…

Q: How resolution in HPLC analysis can be improved?

A: HPLC i.e  high-performance liquid chromatography is an analytical method which is being used to…

Q: What are the similarities between fluorescence detectors in HPLC and chemiluminescence detectors in…

A: Chromatography detectors are used to detect compounds that are eluted from the chromatography…

Q: What is a purification method that is more "green" than flash chromatography? (produces less waste).…

A:

Q: What happen  to the separation raid (RS) in HPLC if I change the mobile phase from methanol/water…

A: Given: We have to tell the effect of polarity on the separation rate in HPLC.

Q: What types of flow meters are used in gas chromatography?

A: ANSWER IS

Q: Explain the term gradient elution mode in HPLC

A: Given : We have to tell about gradient elution term .

Q: SFC combines  important advantages of gas and liquid chromatography. It is especially useful for…

A: The chromatography is the separation technique used for identification and separation of compounds…

Q: Why does silanization reduce tailing of chromatographic peaks?

A: Given observation,  Silanization reduces tailing of chromatographic peaks.

Q: In HPLC, why is purging or initialization being done before analysis of samples, explain.

A: ✓basically following two reasons for using purging and initialization during HPLC sample preparation…

Q: What is the concentration meant by inner filter effects?

A: The concentration for inner filter effects .

Q: pectrofluorometry is potentially more sensitive and selective compared to spectrophotometry

A:

Q: Compare gradient and isocratic mode in HPLC.

A: Gradient and isocratic mode in HPLC are the two different modes of operating condition in HPLC.

Q: select

A: We know that A detector is a device that converts an amount of radiation into some other measurable…

Q: Define, describe, or illustrate the following: a. Selectivity factor b. Resolution factor c. Van…

A: Chromatography is the laboratory technique for the separation and isolation of a variety of samples…

Q: During Size exclusion chromatography, if VT = 30 mL, Vs = 15 cm3 , and VL = 15 mL, Vo is     8 mL…

A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: improve

A: We know that resolution is a measure of how well two Elution peak can be differentiated in…

Q: Two components in an HPLC separation have retention times that differ by 15 s. The first peak elutes…

A: The base number of hypothetical plates under given conditions ought to be resolved to utilize the…

Q: Question 1. Two components in an HPLC separation have retention times that differ by 22 s. The…

A: HPLC ( High performance liquid Chromatography)  It is a useful method for separation of substances…

Q: 4. Enumerate the differences between a serological and a volumetric pipette. 5. Enumerate the uses,…

A: Pipettes are one of the most important instruments in the analytical measurements that help us in…

Q: What is stationary phase used is liquid chromatography? What compounds does it bind best and why?

A: Liquid chromatography is a separation technique on the basis of colour in which the mobile phase is…

Q: A brief introduction on high performance liquid chromatography ( HPLC)

A: It is as follows

Q: High performance liquid chromatography (HPLC) can be used to : a. identify the various pigments from…

A: High performance liquid chromatography (HPLC) is defined as a column chromatography where at high…

Q: What is the purpose of thin layer chromography lab? What is a hypothesis I can use when conducting a…

A: Introduction: Chromatography is a technique used to separate the mixture if solution. There are two…

Q: How we optimize chromatographic separations?

A: Chromatographic separation of mixture of various compounds are based on their distribution between a…

Q: All chromatographic techniques can be used as separation methods. True O False

A: