Which statement best describes the principle behind the succinate dehydrogenase (SDH) assay used in the assay of subcellular fractions from mung bean seedlings?     Question 11 options:   SDH oxidized succinate to fumarate, then fumarate reduced DCPIP.   Succinate is blue in colour, which is eliminated by its oxidation to fumarate by SDH.   SDH produced FADH2, then FADH2 reduced DCPIP.   SDH generated DCPIP, then DCPIP oxidized succinate to fumarate.

distribution of Benzene in a 4-compartment PBK model after an exposure of 6h. Convert Berkeley Madonna code to Matlab.  Note: The parameters in the table below are for a mouse model. 1a) What are the two type of products containing Benzene consumers were recently concerned about. Which of these two different type of products is likely more harmful? (hint: check news/reports and social media channels)   1b) Plot Jresp along with the concentrations of Benzene in blood, fat, non-fat and liver in a 24h window (pdf). Why is Jresp slightly decreasing within 6h of exposure? Here is the Berkeley Madonna code: {Top model}   METHOD RK4   STARTTIME = 0 STOPTIME = 24 DT = 0.02        {Reservoirs Blood, Fat, NonFat and Liver}    d/dt (NF) = + Jnf       INIT NF = 0    d/dt (F) = + Jf       INIT F = 0    d/dt (B) = - Jnf - Jf - Jl + Jresp       INIT B = 0    d/dt (L) = + Jl - Jmetab       INIT L = 0      {Flows}    Jnf = Qnf*(Cb-Cnf)    Jf = Qf*(Cb-Cfv)    Jl = Ql*(Cb - Clv)    Jmetab = vmax*Cl/(Km +…

The above answer did not address the question in the sense that the answer did not indicate which protein involved in the pathways could be measured in the nucleus of cells and be confident in the results? I reiterate the question as follows: You want to do a single Western blot to measure changes in signaling activity, regardless of which pathway is being activated. For which protein involved in these pathways could you measure the levels in the nucleus of cells and be confident in your results? Why?

The progressive loss of cardiac myocytes due to cell death has been associated with cardiac dysfunction and heart failure. Which of the following would lead to a significant loss of cardiac myocytes? Select all that apply Treatment of rapamycin which results in inhibition of mTOR activity Prolonged or severe ER stress with increased accumulation of unfolded proteins Activation of AMPK and phosphorylation of the TSC1 complex mTOR activation and phosphorylation of Ulk1

Please help with this question with a better explanation

Which of the following best describes the production of insulin in by bacterial cells. The biosynthetic pathway that produces human insulin is introduced into bacterial cells on a plasmid. Insulin is purified from bacterial lysates by electrophoresis. Human insulin in a peptide hormone comprised of two chains A and B. Only B chain is required, It is produced by injecting bacteria with MRNA encoding B chain peptide, and purifying the peptides with an antibody. Fuman insulin in a peptide hormone comprised of two chains A and B. It is produced by injecting bacteria with transgenic plasmids containing MRNA encoding either the A and B chain peptides, and purifying the peptides with a mix of antibodies against tne A or B chain. aman nsulina peptide hormone comprised of two chains A and B. They are produced separately as fusion proteins with beta-galactosidase when bacterial cells are transformed with plasmids containing either A or B chain with the lacZ sequence. Once purified they are…

Compare and contrast the function of hsp 70 and hsp 60 chaperones.

A protein (PrX) was heated in vitro to 45C for 15 min, in the presence or absence of Hsp40, Hsc70, and ATP. The amount of PrX in aggregated (P, pellet) and soluble (S) fractions was then quantified (Bars, 1 SD). Based on these data, answer the following questions. In all cases, give brief justification for your answer. a) How does Hsp40 alone influence the solubility of PrX? b) How does Hsc70 alone influence the solubility of PrX? c) Do Hsp40 and Hsc70 co-operate in this experiment? d) Which components combined provide maximal solubility of PrX after heat shock?