Which ONE of the following enzymes would NOT remove keratan sulphate attachments? keratanase keratanase II hyaluronidase N-glycosidase O-glycosidase

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Which ONE of the following enzymes would NOT remove keratan sulphate attachments?
keratanase
keratanase II
hyaluronidase
N-glycosidase
O-glycosidase
Transcribed Image Text:Which ONE of the following enzymes would NOT remove keratan sulphate attachments? keratanase keratanase II hyaluronidase N-glycosidase O-glycosidase
Following PCR amplification of the CYP21 gene from a patient sample, the amplified DNA was digested with a
restriction enzyme (RE) in order to determine the presence of a specific mutation. A normal gene has two cut sites for
the RE, however a mutated gene would show an additional cut site.
Following gel electrophoresis to separate the DNA fragments, how many fragments would you most likely
expect to see if the patient was heterozygous for the mutation?
02
03
4
05
06
07
08
Transcribed Image Text:Following PCR amplification of the CYP21 gene from a patient sample, the amplified DNA was digested with a restriction enzyme (RE) in order to determine the presence of a specific mutation. A normal gene has two cut sites for the RE, however a mutated gene would show an additional cut site. Following gel electrophoresis to separate the DNA fragments, how many fragments would you most likely expect to see if the patient was heterozygous for the mutation? 02 03 4 05 06 07 08
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