Which of the PDH coenzymes does not get restored to its original form at the end of the PDH reaction? FAD TPP NAD+ Lipoamide
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Which of the PDH coenzymes does not get restored to its original form at the end of the PDH reaction?
FAD
TPP
NAD+
Lipoamide
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- The enzyme that catalyzes reaction below can be classified as: CoO COO NAD+ NADH + H* Но- | malate -C- H-Ć- dehydrogenase Охaloacetate Malate isomerase lyase hydrolase oxidoreductase ligase transferaseSome cofactors that participate in the reactions of the citric acid cycle are indicated by letters. Identify the position of positions of NADH in the cycle by selecting the appropriate letter or letters designating its position(s) in the cycle diagram. COA CH, HO--Co, CH čo, 8 CO но-сн CH HC -Co, CO HO-CH LA Citric acid cycle H,0 co, -B COA | 4 Co, čo D.Below is an image of the Krebs cycle: acetyl-CoA oxaloacetate COASH H20 NADH NAD* H20 malate citrate fumarate isocitrate FADH2 NAD* CO2 FAD АТР NADH + ADP succinate GTP NAD+ a-ketoglutarate H20 GDP NADH + CO2 COASH succinyl CoA COASH Consider the conversion of succinate to fumarate, which is coupled with the production the electron carrier FADH2. If this reaction was NOT coupled with the production of FADH2 (and only catalyzed the conversion of succinate to fumarate), how would this impact ATP production through cell respiration? OATP production would stop because no high energy electron carriers would be produced ATP production would still occur, but there would be a much lower ATP yield because a large number of electron carriers are no longer being made ATP production would stop because without FADH2 we will no longer have electrons moving through the electron transport chain ATP production would still occur, but there would be a slightly lower ATP yield because a small number of…
- Which of the following enzymes below is/are involved in the control of Krebs' cycle? citrate synthetase aconitase Fumarase isocitrate dehydrogenaseThe reaction pictured is an oxidation-reduction reaction in the citric acid cycle in which the energy-carrier molecule NADH is generated. Identify which molecule in the reaction will be oxidized and which molecule will be reduced. Place a single answer choice in each box. COO- HO-C-H H-C-H COO- Malate NAD+ NADH + H+ Oxidized malate oxaloacetate COO- H-C-H ī COO- Oxaloacetate Reduced NADH NAD+Which of the following statements inaccurately describes glutamate dehydrogenase? Glutamate dehydrogenase uses either NAD+ or NADP+ in a redox reaction Glutamate dehydrogenase catalyzes an oxidative deamination reaction Glutamate dehydrogenase equilibrium lies with the reductive amination of glutamate Glutamate dehydrogenase utilizes hydrolysis to release ammonium from an imine intermediate
- Which of the following is NOT a cofactor for the pyruvate dehydrogenase complex? Lipoate COA FAD NADP+ All of the above are cofactors for the PDH complex.Malate dehydrogenase is an enzyme that converts malate to oxaloacetate in the last stage of the TCA Cycle (citric acid cycle/Krebs cyde), represented by the following equation: NAD+ Malate Oxaloacetate NADH A group of students carried out an experiment to determine the optimum temperature for the activity of a commercially produced malate dehydrogenase (from yeast) at several different temperatures. A series of test tubes was set up containing 2.0 cm of phosphate buffer with a pH of 7.5, 0.1 cm? of NADH, 0.1 cm of malate dehydrogenase and 0.7 cm3 of water. The tubes were incubated in water baths at the various temperatures for 5 minutes. At certain time intervals each tube was placed in a colorimeter, set at an absorbance value of 1.0. The reaction was then started by adding 0.1 cm? of oxaloacetic acid to the tube. Enzyme activity was measured by following the decrease in absorbance for 120 seconds. The experiment was repeated to give duplicate results. Enzyme activity was then…Which one of the following statements about coenzyme A is correct? The biosynthesis of coenzyme A requires vitamin B12 O The thioester of acetyl-coenzyme A raises the pka value of the alpha-proton O Hydrolysis of acetyl-coenzyme A is thermodynamically favourable O Acetyl-coenzyme A is involved in the biosynthesis of S-adenosylmethoinine
- The PDH mechanism coenzyme that plays a direct role in the generation of acetyl CoA is NADH Lipoamide FADH2 TPPSome anaerobic prokaryotes use nitrate (NO3- ) as the terminal electronacceptor for energy metabolism. Assuming 100% effi ciency, how much ATP could be synthesized by the oxidation of NADH by nitrate?The tricarboxylate transport system supplies cytosolic acetyl-CoA for palmitate synthesis. What percentage of the NADPH required for palmitate synthesis is thereby provided?