Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Which of the following statements about enzymatic catalysis is not true?
a. Enzymes are able to provide different solvent environments at the active site.
O b. Enzymes allow one group to exist in its acidic form while a nearby group can exist in its basic form.
O C. Solvent pH is unimportant in enzymatic reactions.
d. A pH-rate profile plots enzyme activity versus pH.
O e. Enzyme activity depends on the reaction mixture pH.
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Transcribed Image Text:Which of the following statements about enzymatic catalysis is not true? a. Enzymes are able to provide different solvent environments at the active site. O b. Enzymes allow one group to exist in its acidic form while a nearby group can exist in its basic form. O C. Solvent pH is unimportant in enzymatic reactions. d. A pH-rate profile plots enzyme activity versus pH. O e. Enzyme activity depends on the reaction mixture pH.
A decapeptide was analyzed and the following information obtained. Determine the primary structure of the peptide.
Edman's Degradation: PTH-Ala
Trypsin produces 3 peptides of the following composition
1. Gly, His
2. Ala, Leu, Lys, Val
3. Arg (2), Phe, Pro
Chymotrypsin produces 2 peptides of the following composition:
1. Ala, Leu, Lys, Phe, Val
2. Arg (2), Gly, His, Pro
Elastase produces 1 amino acids and 3 peptides
1. Ala
2. Leu, Val
3. Arg (2), Lys, Phe, Pro, Gly, His
-O-C-C
Ala
Arg
Hs
Lys
Phe Pio
Val
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Transcribed Image Text:A decapeptide was analyzed and the following information obtained. Determine the primary structure of the peptide. Edman's Degradation: PTH-Ala Trypsin produces 3 peptides of the following composition 1. Gly, His 2. Ala, Leu, Lys, Val 3. Arg (2), Phe, Pro Chymotrypsin produces 2 peptides of the following composition: 1. Ala, Leu, Lys, Phe, Val 2. Arg (2), Gly, His, Pro Elastase produces 1 amino acids and 3 peptides 1. Ala 2. Leu, Val 3. Arg (2), Lys, Phe, Pro, Gly, His -O-C-C Ala Arg Hs Lys Phe Pio Val
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