Which of the following sequences best describes the correct (5' – 3') organization of a typical eukaryotic gene?   Question 12 options:   TSS - TATA box – enhancer - coding sequence – BREd - termination site   TATA box – TSS – BREd - coding sequence – termination site – enhancer   TATA box – BREd - TSS – operon – termination site – enhancer   TATA box – BREd – TSS – coding sequence – termination site - enhancer   Enhancer -  BREd – TSS – TATA box – coding sequence – termination si

Q: When researchers analyzed the gad operon they found the following: Expression of the gad operon at…

A: Here we saw that mutant 1 is causing the expression of gad protein even at pH7 which was not…

Q: Mediator is not necessary to reproduce transcription initiation reactions in vitro. If you are…

A: In the study of eukaryotic gene expression, understanding the process of transcription initiation is…

Q: Choose the best answer. transcriptional bubble in prokaryotes is produced by… Group of answer…

A: Transcription is the process by which RNA is produced from the DNA template. This process occurs…

Q: Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect…

A: The mutation is the change in the nucleotide sequence of the DNA. The point mutation is the mutation…

Q: Shown below is the genomic structure of the human B-globin gene. The numbers within the boxes…

A: Transcription is the process by which DNA act as template for the formation of mRNA . This process…

Q: For the following questions, describe the expression levels of the structural genes in the Trp…

A: Tryptophan is an essential amino acid, and the trp operon is a genetic regulatory system that can be…

Q: The process in which the two-dimensional structure of RNA from the L region of an operon can either…

A: The strategy of creating an RNA duplicate of a grouping is transcription. The transcript, known as…

Q: Termination of DNA transcription in prokaryotes can be   Question 22 options:   intrinsic or…

A: Termination of transcription is the process by which RNA polymerase-catalyzed RNA synthesis on a DNA…

Q: Complete Table 1, indicating how much (lots, little, none) of the above gene product would be made…

A: An operon is a single transcription unit that can include many genes that code for different…

Q: Liquid condensates may be a useful physico-chemical property of the overall transcription reaction.…

A: Liquid condensates or biomolecular condensates are dynamic, micron-scale compartments in eukaryotic…

Q: For the following gene, which type of regulatory sequence has likely been deleted in mutant 1?…

A: Transcription is the mechanism by which an RNA copy of a gene sequence is produced. This clone,…

Q: The trp operon in E. coli encodes enzymes essential for the biosynthesis of tryptophan. In the…

A: trp operon is a repressor operon found in E. coli bacteria, which consists of a group of genes that…

Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase…

A: Inactivating mutations, also known as loss-of-function mutations, cause the gene product to have…

Q: that best fits the phrase below (1-20) Cis-acting B Post-transcriptional modification…

A: Francis Crick proposed central dogma. The RNA is formed from DNA with the help of enzyme RNA…

Q: Which of the following DNA regions Is NOT Involved in gene expressi regulation in eukaryotes?

A: Ans- Operators are not involved in the Eukaryotic Gene Expression. Eukaryotic protein-coding genes…

Q: Which of the following is NOT a cis-acting element involved in eukaryotic transcription?   Question…

A: Cis- acting regulatory sequence in DNA are that control a gene only on the same chromosome. In…

Q: After comparing the whole genome sequencing results from a candidate that you suspect has a mutation…

A: A missense mutation is a type of genetic mutation that results in the substitution of one nucleotide…

Q: control medium that demonstrates the general growth rate of mutant candidates

A: Control Medium: This medium is used to demonstrate the general growth rate of mutant candidates in…

Q: A eukaryotic cDNA was ligated to a phage DNA which was transfected into a bacterial host cell. The…

A: The cDNA is produced from the mature mRNA by the action of reverse transcriptase enzyme.

Q: You are teaching a class on the regulation of eukaryotic gene expression. such as the promoter…

A: Binding of RNA polymerase II  and transcription factors is necessary for transcription.

Q: You made four mutants for a promoter sequence in DNA and studied them for transcription. The results…

A: Promoter are the sequence present in the DNA. These allow the binding of transcription factor and…

Q: RNA-induced gene silencing can be accomplished by cleaving and degrading mRNA encoding for a…

A: The control of gene expression is far more complex in eukaryotes than in prokaryotes. the gene…

Q: How does the sigma factor of E. coli RNA polymerase bind to promoters? Question 14 options: a…

A: Sigma factors direct the binding of RNA polymerase to promoters. It places the enzyme in a position…

Q: Please match the gene element with the the correct definition. control elements 5' UTR 3' UTR…

A: The process by which genetic information encoded in DNA is copied into RNA is known as…

Q: What are the operon fusion and the gene fusion? How can they be used for studying gene expression in…

A: Fusion gene is a hybrid gene formed from two previously independent genes or ORFs (Open Reading…

Q: 1) The UR operon which is composed of sequences A, B, C, and D (which may or may not be genes of…

A: An operon may be considered as a group of certain genes which function together so as to regulate…

Q: 5) The bacterial Lac operon is an example of transcriptional regulation. What are the major…

A: Using an example of Escherichia coli for the bacterial lac operon. Escherichia coli is a…

Q: List and describe all the major players involved in transcription of the lac operon in bacteria…

A: Lactose operon is an example of inducible operon and it is the gene expression regulatory mechanism…

Q: The following is a polypeptide from a wild type organism: met – ser – tyr – his – trp – arg – ser –…

A: Mutation is any sudden change resulting in an abnormal structure.  The given polypeptide chain is,…

Q: 15a) Most amino acid biosynthetic operons in prokaryotes have a structure similar to that observed…

A: The tryptophan operon consists of five structural genes: trpE, trpD, trpC, trpB, trpA that codes for…

Q: Which of the following locations would NOT be a possible site for an enhancer for a gene in a…

A: A gene is a unit of heredity that is responsible for the transmission of a specific trait or…

Q: How does the sigma factor of E. coli RNA polymerase bind to promoters? Question 10 options: a…

A: Two grooves of DNA are formed by an antiparallel arrangement of DNA strands. They are sites for…

Q: Let’s suppose a DNA mutation changes the consensus sequence at the −35 site in a way that inhibits σ…

A: A sigma factor or specificity factor refers to the protein factor required for the transcription…

Q: Complete the table below by choosing a term(s) in Column B that associates with the term in Column…

A: The repressor, LacI, binds to the operator sequence and prevents transcription of those genes unless…

Q: Which of the following are the enzymes involved in post-transcriptional modification?   Question…

A: The objective of the question is to identify the enzymes involved in post-transcriptional…

Q: A mutation in which of the following would still allow RNA polymerase to bind to promoters but would…

A: To start transcribing a gene, RNA polymerase binds to the promoter region of the gene's DNA. It has…

Q: From the list given - choose all the regulatory sequences that you think would control the…

A: A regulatory sequence is a nucleic acid molecule segment that has the ability to increase or…

Q: Which of the following is characteristic of an RNA polymerase II in the elongation phase of…

A: From DNA,mRNA is formed by RNA polymerase and this process is called as transcription process.In…

Q: Which of the follow general transcription factors contains the protein TBP and binds to TATA box…

A: The correct answer is: a. TFIID TFIID is a general transcription factor that contains the…

Q: Identify the DNA elements and protein factors, #1- 7, in the figure below that are involved in the…

A: Central Dogma =  According to the central DNA is converted to RNA (transcription) and RNA to protein…

Q: Assuming that the level of glucose is low, a mutation in the repressor associated with the lac…

A: The lac operon which is also known as lactose operon is a set of genes that are specific for uptake…

Q: After comparing the whole genome sequencing results from a candidate that you suspect has a mutation…

A: A missense mutation is a type of genetic mutation that causes a change in a single nucleotide of the…

Q: Usually found in prokaryotes, regulatory sequences lying adjacent to the DNA being transcribed are…

A: Usually found in prokaryotes, regulatory sequences lying adjacent to the DNA being transcribed are…

Q: 9. Give the levels of Beta-galactosidase activity (high or low or zero) expected for the following…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: Matching type Choices are in the picture   11. regulating elements in the operon 12. ribosome…

A: Transcription is the process by which the information in a strand of DNA is copied into a new…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.