Which of the following mobile genetic elements is a retrotransposon, making up about 10% of the human genome, but no longer codes for a functional gene product (and is therefore a “pseudogene”)? the LINE-1 element (repeated up to 50,000 times in the human genome) the DNA-only transposons known as “jumping genes” (in corn) the avocado sunblotch viroid the typical transposon found in a bacterial chromosome the Alu sequence (repeated up to 1,000,000 times in the human genome)

The following image shows the 'beads-on-a-string' model of DNA. Where is Gene 1 located? Gene 1 Ac Ac (Ac (Ac O In the promoter DNA O In a nucleosome core particle O In the linker DNA O On a histone

Which of the following statements about genomic libraries and cDNA libraries is TRUE? O a genomic library contains exons only O a cDNA library is derived from chromosomal DNA O a genomic library is made using reverse transcriptase O a genomic library is derived from mRNA O a cDNA library is derived from mRNA and is made using reverse transcriptase

What portions of the above genomic sequence actually code for amino acids?

Which of the following observations give support to the idea that changes in chromatin structure affect gene activity? nucleosome removal near active genes 0000 position effect variegation formation fo Barr bodies in mammals all of the above

Dystrophin is a protein that forms part of a vital protein complex that connects the cytoskeleton of a muscle fiber cell to the extracellular matrix. This connection strengthens and shapes the muscle fibers. Dystrophin is coded by the DMD gene. This is one of the longest human genes known, covering 2,300,000 base pairs (0.08% of the human genome) It is located in chromosome 21. The immature mRNA is 2,100,000 bases long and takes 16 hours to transcribe. It contains 79 exons. The mature mRNA measures 14,000 and codes for a protein with 3,685 amino acids. Abnormal expression of dystrophin leads to severe symptoms like muscle weakness and fatigability, a disease that is called muscular dystrophy. Most patients with muscular dystrophy become wheelchair dependent early in life. Cardiac muscle is also affected which results typically in premature death (~ second or third decade of life). Several mutations in this gene have led to the production of low levels of dystrophin or of a defective,…

Below is a portion of an exon from a gene that encodes protein X in the genome of the plant Arabidopsis. Wildtype DNA3’ TTC  AAT GCT CCG AAT ACC 5’ template strand5’ AAG TTA CGA GGC TTA TGG 3’ non-template strand A new strain (Strain B) of Arabidopsis is identified with the same region of the gene coding for protein X: 3’ TTC  AAT GCT CCC AAT ACC 5’ template strand5’ AAG TTA CGA GGG TTA TGG 3’ non-template strand Compare the two DNA sequences and look for any differences. Based on what you find   a. There is no mutation in Strain B compared to Strain A. b. After the point of the mutation, all the amino acids encoded by the Strain B template will be different than the Strain A protein X. c. Protein X made from the Strain B template will be much shorter than protein X made from the Strain A template d. Protein X from Strain B will have one amino acid difference that would not affect protein function. e. There is a mutation but there will not be any difference in the…

Wild type genomic sequence of a portion of a gene and the wild type sequence of portion of the protein gene product. The protein sequence only matches partially. TAT AAA GGG CGA CCA CCT GGT AAT GGG ACT TTG AGG Tyr Lys Gly Arg Pro Pro Ala Pro Arg Gln Tyr Trp Note: The five amino acids at the amino end of the WT protein match the coding sequence perfectly, but not the six at the carboxyl end. This is for an obvious reason that you should figure out before starting the problem. One of the difficulties with this problem is that you do not have the DNA sequence for the carboxyl end of the protein, but that should not be a problem! And, so far, there are no mutations; nothing unusual, it is just a normal eukaryotic gene!! A mutant is found that has the following protein sequence in that part of the protein: Tyr Lys Gly Arg Thr Cys Ser Premature Stop. What is the likely mutation? between A12 and C13 insert T. O between C13 and C14 insert T. A15 -> del. O C13 and C14 -> del. G8 -> T. OG20 ->…

Hello there, A little confused about if a superrepressor can act on a constitutive operator. Thanks in advance!

Below is a portion of an exon from a gene that encodes protein Y in the genome of the plant Brassica. Wildtype DNA3’ CTT  AAT GCT CCG AAT CCA 5’ template strand5’ GAA TTA CGA GGC TTA GGT 3’ non-template strand A new strain (Strain X) of Brassica is identified with the same region of the gene coding for protein Y:3’ CTT  AAT GCT GCG AAT CCA 5’ template strand5’ GAA TTA CGA CGC TTA GGT 3’ non-template strand   Compare the sequence of Wildtype with Strain X DNA, and note the following: Whether there is a mutation. If there is a mutation, what is the type of mutation (be as specific as possible) and explain the rationale for your decision.  Assuming this is the only difference between the Wildtype and Strain X, describe the potential impact of the mutation on the structure and function of the protein.